Lengheden A, Jansson L
Department of Oral Histology and Cell Biology, School of Dentistry, Karolinska Institute, Huddinge, Sweden.
Eur J Oral Sci. 1995 Jun;103(3):148-55. doi: 10.1111/j.1600-0722.1995.tb00016.x.
The aim of the present study was to examine to what extent pH affects human fibroblast repopulation of an experimental wound in vitro laying special emphasis on cell migration, DNA synthesis, and alterations in cell morphology. Wounds were simulated in vitro by partially scraping off monolayers of human embryonic diploid lung fibroblast and human periodontal ligament fibroblast-like cells respectively. The wounded monolayers were cultured at pHs ranging from 7.2 to 8.4. The experiments were evaluated by autoradiography as well as light and scanning electron microscopic techniques. A significant, approximately linear, decrease was noted both in cell migration into and DNA synthesis in the experimental wounds as a result of pH increase. The surviving cells in the wounds showed cytoplasmatic vacuoles and blebbing at pH levels above 7.8. Clinical implications of these results for endodontic calcium hydroxide treatment are that hydroxyl ions, diffusing through root dentin, may interfere with vital cell functions necessary for healing on the root surface.
本研究的目的是探讨pH值在多大程度上影响体外实验性伤口中人类成纤维细胞的再填充情况，特别关注细胞迁移、DNA合成以及细胞形态的改变。通过分别部分刮除人胚胎二倍体肺成纤维细胞和人牙周膜成纤维样细胞的单层来在体外模拟伤口。将受伤的单层细胞在pH值范围为7.2至8.4的条件下培养。通过放射自显影以及光学和扫描电子显微镜技术对实验进行评估。由于pH值升高，实验伤口中的细胞迁移和DNA合成均出现显著的、近似线性的下降。伤口中存活的细胞在pH值高于7.8时出现细胞质空泡和起泡现象。这些结果对牙髓氢氧化钙治疗的临床意义在于，通过牙本质扩散的氢氧根离子可能会干扰牙根表面愈合所需的重要细胞功能。
