不同的内源性代谢物可触发酿酒酵母中糖酵解基因表达的诱导。
Different internal metabolites trigger the induction of glycolytic gene expression in Saccharomyces cerevisiae.
作者信息
Müller S, Boles E, May M, Zimmermann F K
机构信息
Institut für Mikrobiologie, Technische Hochschule Darmstadt, Germany.
出版信息
J Bacteriol. 1995 Aug;177(15):4517-9. doi: 10.1128/jb.177.15.4517-4519.1995.
Abstract
In the yeast Saccharomyces cerevisiae, the sugar-induced expression of various genes coding for glycolytic enzymes is triggered by increases in the concentrations of different internal metabolites. Here, we show that the induction of the glycolytic isoenzyme enolase 2 is strictly dependent on the abilities of different mutant strains to increase the level of glucose-6-phosphate after the addition of sugars. In contrast, the induction of alcohol dehydrogenase I is dependent on increasing concentrations of metabolites in the late stages of glycolysis.
摘要
在酿酒酵母中，编码糖酵解酶的各种基因的糖诱导表达是由不同内部代谢物浓度的增加引发的。在这里，我们表明糖酵解同工酶烯醇化酶2的诱导严格依赖于不同突变菌株在添加糖后提高6-磷酸葡萄糖水平的能力。相比之下，乙醇脱氢酶I的诱导依赖于糖酵解后期代谢物浓度的增加。
相似文献
1
Different internal metabolites trigger the induction of glycolytic gene expression in Saccharomyces cerevisiae.不同的内源性代谢物可触发酿酒酵母中糖酵解基因表达的诱导。
J Bacteriol. 1995 Aug;177(15):4517-9. doi: 10.1128/jb.177.15.4517-4519.1995.
2
The glucose-6-phosphate-isomerase reaction is essential for normal glucose repression in Saccharomyces cerevisiae.葡萄糖-6-磷酸异构酶反应对于酿酒酵母中的正常葡萄糖阻遏至关重要。
Eur J Biochem. 1993 May 15;214(1):121-7. doi: 10.1111/j.1432-1033.1993.tb17903.x.
3
Different signals control the activation of glycolysis in the yeast Saccharomyces cerevisiae.不同的信号控制酿酒酵母中糖酵解的激活。
Yeast. 1993 Jul;9(7):761-70. doi: 10.1002/yea.320090710.
4
Overproduction of glycolytic enzymes in yeast.酵母中糖酵解酶的过量产生。
Yeast. 1989 Jul-Aug;5(4):285-90. doi: 10.1002/yea.320050408.
5
Structure and expression of yeast glycolytic genes.
Basic Life Sci. 1982;19:291-303. doi: 10.1007/978-1-4684-4142-0_23.
6
Analysis of transcription and translation of glycolytic enzymes in glucose-limited continuous cultures of Saccharomyces cerevisiae.酿酒酵母葡萄糖限制连续培养中糖酵解酶转录与翻译的分析
J Gen Microbiol. 1992 Dec;138(12):2559-66. doi: 10.1099/00221287-138-12-2559.
7
Molecular cloning of a gene involved in glucose sensing in the yeast Saccharomyces cerevisiae.参与酿酒酵母葡萄糖感应的一个基因的分子克隆。
Mol Microbiol. 1993 May;8(5):927-43. doi: 10.1111/j.1365-2958.1993.tb01638.x.
8
Identification of a regulatory region that mediates glucose-dependent induction of the Saccharomyces cerevisiae enolase gene ENO2.鉴定介导葡萄糖依赖性诱导酿酒酵母烯醇化酶基因ENO2的调控区域。
Mol Cell Biol. 1986 Jul;6(7):2287-97. doi: 10.1128/mcb.6.7.2287-2297.1986.
9
The GCR1 gene encodes a positive transcriptional regulator of the enolase and glyceraldehyde-3-phosphate dehydrogenase gene families in Saccharomyces cerevisiae.GCR1基因编码酿酒酵母中烯醇酶和甘油醛-3-磷酸脱氢酶基因家族的一个正向转录调节因子。
Mol Cell Biol. 1987 Feb;7(2):813-20. doi: 10.1128/mcb.7.2.813-820.1987.
10
Global Metabolic Engineering of Glycolytic Pathway via Multicopy Integration in Saccharomyces cerevisiae.通过多拷贝整合在酿酒酵母中对糖酵解途径进行全局代谢工程改造
ACS Synth Biol. 2017 Apr 21;6(4):659-666. doi: 10.1021/acssynbio.6b00281. Epub 2017 Jan 23.
引用本文的文献
1
Metabolomic and (13)C-metabolic flux analysis of a xylose-consuming Saccharomyces cerevisiae strain expressing xylose isomerase.表达木糖异构酶的木糖消耗型酿酒酵母菌株的代谢组学和(13)C代谢通量分析。
Biotechnol Bioeng. 2015 Mar;112(3):470-83. doi: 10.1002/bit.25447. Epub 2014 Nov 24.
2
Dynamic metabolomics differentiates between carbon and energy starvation in recombinant Saccharomyces cerevisiae fermenting xylose.动态代谢组学可区分重组酿酒酵母发酵木糖过程中的碳饥饿和能量饥饿。
Biotechnol Biofuels. 2012 May 15;5(1):34. doi: 10.1186/1754-6834-5-34.
3
Limitations in xylose-fermenting Saccharomyces cerevisiae, made evident through comprehensive metabolite profiling and thermodynamic analysis.通过全面的代谢物分析和热力学分析，揭示了木糖发酵酿酒酵母的局限性。
Appl Environ Microbiol. 2010 Nov;76(22):7566-74. doi: 10.1128/AEM.01787-10. Epub 2010 Oct 1.
4
Cross-reactions between engineered xylose and galactose pathways in recombinant Saccharomyces cerevisiae.重组酿酒酵母中工程化木糖和半乳糖途径之间的交叉反应。
Biotechnol Biofuels. 2010 Sep 1;3:19. doi: 10.1186/1754-6834-3-19.
5
The glucose signal and metabolic p[H+]lux.葡萄糖信号与代谢性[H⁺]通量。
EMBO J. 2010 Aug 4;29(15):2473-4. doi: 10.1038/emboj.2010.161.
6
PGM2 overexpression improves anaerobic galactose fermentation in Saccharomyces cerevisiae.过表达 PGM2 可提高酿酒酵母的厌氧半乳糖发酵性能。
Microb Cell Fact. 2010 May 27;9:40. doi: 10.1186/1475-2859-9-40.
7
Switching the mode of metabolism in the yeast Saccharomyces cerevisiae.改变酿酒酵母的代谢模式。
EMBO Rep. 2004 May;5(5):532-7. doi: 10.1038/sj.embor.7400132. Epub 2004 Apr 8.
8
Regulation of glycolysis in Kluyveromyces lactis: role of KlGCR1 and KlGCR2 in glucose uptake and catabolism.乳酸克鲁维酵母中糖酵解的调控：KlGCR1和KlGCR2在葡萄糖摄取和分解代谢中的作用。
Curr Genet. 2004 Mar;45(3):129-39. doi: 10.1007/s00294-003-0473-5. Epub 2003 Dec 19.
9
Molecular analysis of a Saccharomyces cerevisiae mutant with improved ability to utilize xylose shows enhanced expression of proteins involved in transport, initial xylose metabolism, and the pentose phosphate pathway.对一株利用木糖能力有所提高的酿酒酵母突变体进行分子分析，结果显示参与转运、木糖初步代谢及磷酸戊糖途径的蛋白质表达增强。
Appl Environ Microbiol. 2003 Feb;69(2):740-6. doi: 10.1128/AEM.69.2.740-746.2003.
10
Anaerobic xylose fermentation by recombinant Saccharomyces cerevisiae carrying XYL1, XYL2, and XKS1 in mineral medium chemostat cultures.在矿物培养基恒化器培养中，携带XYL1、XYL2和XKS1的重组酿酒酵母进行厌氧木糖发酵。
Appl Environ Microbiol. 2000 Aug;66(8):3381-6. doi: 10.1128/AEM.66.8.3381-3386.2000.
本文引用的文献
1
Saccharomyces cerevisiae phosphoglucose isomerase and fructose bisphosphate aldolase can be replaced functionally by the corresponding enzymes of Escherichia coli and Drosophila melanogaster.酿酒酵母磷酸葡萄糖异构酶和果糖二磷酸醛缩酶在功能上可被大肠杆菌和黑腹果蝇的相应酶替代。
Curr Genet. 1993 Mar;23(3):187-91. doi: 10.1007/BF00351494.
2
Different signals control the activation of glycolysis in the yeast Saccharomyces cerevisiae.不同的信号控制酿酒酵母中糖酵解的激活。
Yeast. 1993 Jul;9(7):761-70. doi: 10.1002/yea.320090710.
3
Induction of pyruvate decarboxylase in glycolysis mutants of Saccharomyces cerevisiae correlates with the concentrations of three-carbon glycolytic metabolites.酿酒酵母糖酵解突变体中丙酮酸脱羧酶的诱导与三碳糖酵解代谢物的浓度相关。
Arch Microbiol. 1993;160(4):324-8. doi: 10.1007/BF00292085.
4
The role of the NAD-dependent glutamate dehydrogenase in restoring growth on glucose of a Saccharomyces cerevisiae phosphoglucose isomerase mutant.NAD 依赖型谷氨酸脱氢酶在酿酒酵母磷酸葡萄糖异构酶突变体的葡萄糖生长恢复中的作用。
Eur J Biochem. 1993 Oct 1;217(1):469-77. doi: 10.1111/j.1432-1033.1993.tb18266.x.
5
Sequence and functional analysis of a 7.2 kb fragment of Saccharomyces cerevisiae chromosome II including GAL7 and GAL10 and a new essential open reading frame.酿酒酵母II号染色体7.2 kb片段的序列和功能分析，该片段包含GAL7和GAL10以及一个新的必需开放阅读框。
Yeast. 1995 Jan;11(1):79-83. doi: 10.1002/yea.320110110.
6
7
Construction and use of gene fusions to lacZ (beta-galactosidase) that are expressed in yeast.构建并使用在酵母中表达的与lacZ（β-半乳糖苷酶）的基因融合体。
Methods Enzymol. 1983;101:167-80. doi: 10.1016/0076-6879(83)01012-5.
8
A kinetic study of glycolytic enzyme synthesis in yeast.酵母中糖酵解酶合成的动力学研究。
J Biol Chem. 1971 Jan 25;246(2):475-88.
9
Control of glycolytic enzyme synthesis in yeast by products of the hexokinase reaction.己糖激酶反应产物对酵母糖酵解酶合成的调控
J Biol Chem. 1971 Jan 25;246(2):489-99.
10
Identification of a regulatory region that mediates glucose-dependent induction of the Saccharomyces cerevisiae enolase gene ENO2.鉴定介导葡萄糖依赖性诱导酿酒酵母烯醇化酶基因ENO2的调控区域。
Mol Cell Biol. 1986 Jul;6(7):2287-97. doi: 10.1128/mcb.6.7.2287-2297.1986.
Zotero 插件