Briars G L, Dean T P, Murphy J L, Rolles C J, Warner J O
Department of Paediatric Medicine, Southampton General Hospital.
Arch Dis Child. 1995 Jul;73(1):74-6. doi: 10.1136/adc.73.1.74.
Interleukin-8 (IL-8) and tumour necrosis factor-alpha (TNF-alpha) concentrations were measured in faecal samples from nine patients with cystic fibrosis and nine healthy age matched controls. The patients were assessed with Shwachman score, apparent energy absorption, pancreatic enzyme dosage, simple spirometry, and presence of pseudomonal colonisation. Median (range) wet stool IL-8 and TNF-alpha concentrations in patients were 32,113 pg/g (21,656-178,128) and 3187 pg/g (368-17,611) respectively, compared with < 43.5 pg (IL-8)/g (< 22-4079) and 99 pg (TNF-alpha)/g (< 0.26-231) in controls. IL-8 concentration was negatively correlated with Shwachman score (r = -0.79) and pancreatic enzyme dosage (r = -0.77), but not with energy absorption. Seven patients were mature enough to cooperate with spirometry. Their IL-8 concentrations correlated with percentage predicted forced expiratory volume in one second (r = -0.78). IL-8 concentration was greater in four patients with, than five without, established pseudomonal colonisation: median difference 134,583 pg/g. TNF-alpha concentration was not correlated with measures of disease severity. Faecal IL-8 concentration might reflect the severity of pulmonary inflammation in cystic fibrosis and could provide an easily obtainable marker of disease activity.
在9名囊性纤维化患者和9名年龄匹配的健康对照者的粪便样本中检测了白细胞介素-8（IL-8）和肿瘤坏死因子-α（TNF-α）的浓度。通过施瓦克曼评分、表观能量吸收、胰酶剂量、简易肺活量测定法以及铜绿假单胞菌定植情况对患者进行评估。患者粪便中IL-8和TNF-α浓度的中位数（范围）分别为32,113 pg/g（21,656 - 178,128）和3187 pg/g（368 - 17,611），而对照组分别为< 43.5 pg（IL-8）/g（< 22 - 4079）和99 pg（TNF-α）/g（< 0.26 - 231）。IL-8浓度与施瓦克曼评分（r = -0.79）和胰酶剂量（r = -0.77）呈负相关，但与能量吸收无关。7名患者年龄足够大，能够配合肺活量测定。他们的IL-8浓度与一秒用力呼气量预测值百分比相关（r = -0.78）。在已确定有铜绿假单胞菌定植的4名患者中，IL-8浓度高于未定植的5名患者：中位数差异为134,583 pg/g。TNF-α浓度与疾病严重程度指标无关。粪便IL-8浓度可能反映囊性纤维化患者肺部炎症的严重程度，并可为疾病活动提供一个易于获取的标志物。
