King B L, Carcangiu M L, Carter D, Kiechle M, Pfisterer J, Pfleiderer A, Kacinski B M
Department of Therapeutic Radiology, Yale University School of Medicine, New Haven, Connecticut 06520, USA.
Br J Cancer. 1995 Aug;72(2):376-82. doi: 10.1038/bjc.1995.341.
Microsatellite instability has been observed in a variety of sporadic malignancies, but its existence in sporadic ovarian cancer has been the subject of conflicting reports. We have performed a polymerase chain reaction-based microsatellite analysis of DNAs extracted from the neoplastic and non-neoplastic tissues of 41 ovarian cancer patients. Tumour-associated alterations were observed in seven (17%) of these cases. Clinicopathological correlations revealed that: (1) alterations among tumours classified as serous adenocarcinomas occurred with relatively low frequency (2/24 or 8%); (2) most of the tumours with microsatellite alterations (5/7 or 71%) were of less common histopathological types (epithelial subtypes such as endometrioid and mixed serous and mucinous, or non-epithelial types such as malignant mixed Müllerian or germ cell tumours); (3) tumour-associated alterations were observed in 3/4 (75%) of the patients with stage I tumours vs 4/37 (11%) of the patients with stage II, III and IV tumours (P = 0.01); (4) tumour-associated microsatellite instability was found to occur with similar frequencies among patients with and without clinical features suggestive of familial disease, including positive family history, early onset, or multiple primary tumours. In summary, we have observed microsatellite alterations in the neoplastic tissues of ovarian cancer patients with diverse genetic backgrounds and clinicopathological features. The pattern of alterations is consistent with the possibility that multiple mechanisms may be responsible for microsatellite instability in ovarian neoplasms.
微卫星不稳定性已在多种散发性恶性肿瘤中被观察到，但其在散发性卵巢癌中的存在一直是相互矛盾报道的主题。我们对41例卵巢癌患者的肿瘤组织和非肿瘤组织提取的DNA进行了基于聚合酶链反应的微卫星分析。在其中7例（17%）病例中观察到与肿瘤相关的改变。临床病理相关性显示：（1）在分类为浆液性腺癌的肿瘤中，改变发生的频率相对较低（2/24或8%）；（2）大多数有微卫星改变的肿瘤（5/7或71%）是较不常见的组织病理学类型（上皮亚型，如子宫内膜样以及浆液性和黏液性混合类型，或非上皮类型，如恶性混合苗勒管肿瘤或生殖细胞肿瘤）；（3）I期肿瘤患者中有3/4（75%）观察到与肿瘤相关的改变，而II、III和IV期肿瘤患者中为4/37（11%）（P = 0.01）；（4）在有和没有提示家族性疾病临床特征（包括阳性家族史、早发或多原发性肿瘤）的患者中，发现肿瘤相关微卫星不稳定性的发生频率相似。总之，我们在具有不同遗传背景和临床病理特征的卵巢癌患者的肿瘤组织中观察到了微卫星改变。改变模式与多种机制可能导致卵巢肿瘤微卫星不稳定性的可能性一致。
