Nijweide P J, Modderman W E, Hagenaars C E
Department of Cell Biology, Faculty of Medicine, Leiden University, The Netherlands.
Clin Orthop Relat Res. 1995 Apr(313):92-102.
Many or perhaps all cell types in the body possess extracellular binding sites for nucleosides and nucleotides, the purinoceptors. The binding sites that favor adenosine triphosphate (ATP) have been classified as P2-purinoceptors. One subclass of the P2-purinoceptors is the P2z-purinoceptor that mediates the permeabilizing effect of ATP4- (the fully ionized form of adenosine triphosphate). In the presence of millimolar concentrations of ATP4-, this receptor, which was found on all cells of hemopoietic origin but not on cells of stromal origin, renders the sensitive cells permeable for molecules up to 1 kD. This property has been used to eliminate cells of hemopoietic origin from mixed populations. Skeletal- and blood-forming tissues have a complex cellular composition of predominantly stromal and hemopoietic cells. The 2 cell types influence each others' formation, differentiation, and activities in a largely unclarified manner. Rigorous separation would help the study of the properties and potencies of the 2 cell types and their mutual interactions. A short treatment of cell populations isolated from bone, bone marrow, or cartilage with 2 mM adenosine triphosphate and 1 mM of cytotoxic, but not permeant, potassium thiocyanate obliterates all cells of hemopoietic origin, including macrophages, osteoclasts, and their progenitors from these populations.
体内许多细胞类型（或许所有细胞类型）都拥有核苷和核苷酸的细胞外结合位点，即嘌呤受体。对三磷酸腺苷（ATP）具有偏好性的结合位点已被归类为P2嘌呤受体。P2嘌呤受体的一个亚类是P2z嘌呤受体，它介导ATP4-（三磷酸腺苷的完全电离形式）的通透作用。在毫摩尔浓度的ATP4-存在下，这种在所有造血来源细胞上发现但不在基质来源细胞上发现的受体，使敏感细胞对分子量高达1kD的分子具有通透性。这一特性已被用于从混合群体中清除造血来源的细胞。骨骼和造血组织具有主要由基质细胞和造血细胞组成的复杂细胞构成。这两种细胞类型在很大程度上以未明确的方式相互影响彼此的形成、分化和活性。严格分离将有助于研究这两种细胞类型的特性和效能及其相互作用。用2 mM三磷酸腺苷和1 mM具有细胞毒性但不能通透的硫氰酸钾对从骨骼、骨髓或软骨中分离出的细胞群体进行短暂处理，可清除这些群体中所有造血来源的细胞，包括巨噬细胞、破骨细胞及其祖细胞。
