通过实验进化在铜绿假单胞菌PAO中出现尼龙低聚物降解酶。
Emergence of nylon oligomer degradation enzymes in Pseudomonas aeruginosa PAO through experimental evolution.
作者信息
Prijambada I D, Negoro S, Yomo T, Urabe I
机构信息
Department of Biotechnology, Faculty of Engineering, Osaka University, Japan.
出版信息
Appl Environ Microbiol. 1995 May;61(5):2020-2. doi: 10.1128/aem.61.5.2020-2022.1995.
Abstract
Through selective cultivation with 6-aminohexanoate linear dimer, a by-product of nylon-6 manufacture, as the sole source of carbon and nitrogen, Pseudomonas aeruginosa PAO, which initially has no enzyme activity to degrade this xenobiotic compound, was successfully expanded in its metabolic ability. Two new enzyme activities, 6-aminohexanoate cyclic dimer hydrolase and 6-aminohexanoate dimer hydrolase, were detected in the adapted strains.
摘要
通过以尼龙6制造的副产物6-氨基己酸线性二聚体作为唯一碳源和氮源进行选择性培养，最初对这种外源化合物没有降解酶活性的铜绿假单胞菌PAO的代谢能力得到了成功扩展。在适应菌株中检测到了两种新的酶活性，即6-氨基己酸环状二聚体水解酶和6-氨基己酸二聚体水解酶。
相似文献
1
Emergence of nylon oligomer degradation enzymes in Pseudomonas aeruginosa PAO through experimental evolution.通过实验进化在铜绿假单胞菌PAO中出现尼龙低聚物降解酶。
Appl Environ Microbiol. 1995 May;61(5):2020-2. doi: 10.1128/aem.61.5.2020-2022.1995.
2
Biodegradation of nylon oligomers.尼龙低聚物的生物降解
Appl Microbiol Biotechnol. 2000 Oct;54(4):461-6. doi: 10.1007/s002530000434.
3
Plasmid dependence of Pseudomonas sp. strain NK87 enzymes that degrade 6-aminohexanoate-cyclic dimer.降解6-氨基己酸环状二聚体的假单胞菌属NK87菌株酶的质粒依赖性
J Bacteriol. 1989 Jun;171(6):3181-6. doi: 10.1128/jb.171.6.3181-3186.1989.
4
6-Aminohexanoate oligomer hydrolases from the alkalophilic bacteria Agromyces sp. strain KY5R and Kocuria sp. strain KY2.来自嗜碱细菌土壤杆菌属菌株KY5R和考克氏菌属菌株KY2的6-氨基己酸酯寡聚体水解酶
Appl Environ Microbiol. 2007 Nov;73(21):7099-102. doi: 10.1128/AEM.00777-07. Epub 2007 Sep 7.
5
Nylon oligomer degradation gene, nylC, on plasmid pOAD2 from a Flavobacterium strain encodes endo-type 6-aminohexanoate oligomer hydrolase: purification and characterization of the nylC gene product.来自黄杆菌菌株的质粒pOAD2上的尼龙低聚物降解基因nylC编码内切型6-氨基己酸低聚物水解酶：nylC基因产物的纯化与特性分析
Appl Environ Microbiol. 1993 Nov;59(11):3978-80. doi: 10.1128/aem.59.11.3978-3980.1993.
6
Structural and functional characterization of nylon hydrolases.尼龙水解酶的结构与功能特性研究。
Methods Enzymol. 2021;648:357-389. doi: 10.1016/bs.mie.2020.11.004. Epub 2020 Dec 29.
7
A new nylon oligomer degradation gene (nylC) on plasmid pOAD2 from a Flavobacterium sp.来自黄杆菌属某菌株的质粒pOAD2上的一个新的尼龙低聚物降解基因（nylC）
J Bacteriol. 1992 Dec;174(24):7948-53. doi: 10.1128/jb.174.24.7948-7953.1992.
8
X-ray crystallographic analysis of 6-aminohexanoate-dimer hydrolase: molecular basis for the birth of a nylon oligomer-degrading enzyme.6-氨基己酸二聚体水解酶的X射线晶体学分析：一种尼龙低聚物降解酶诞生的分子基础。
J Biol Chem. 2005 Nov 25;280(47):39644-52. doi: 10.1074/jbc.M505946200. Epub 2005 Sep 14.
9
A plasmid encoding enzymes for nylon oligomer degradation: nucleotide sequence and analysis of pOAD2.一种编码尼龙低聚物降解酶的质粒：pOAD2的核苷酸序列及分析
Microbiology (Reading). 1995 Oct;141 ( Pt 10):2585-90. doi: 10.1099/13500872-141-10-2585.
10
Metabolic pathway of 6-aminohexanoate in the nylon oligomer-degrading bacterium Arthrobacter sp. KI72: identification of the enzymes responsible for the conversion of 6-aminohexanoate to adipate.6-氨基己酸在尼龙寡聚物降解菌节杆菌属 KI72 中的代谢途径：鉴定负责将 6-氨基己酸转化为己二酸的酶。
Appl Microbiol Biotechnol. 2018 Jan;102(2):801-814. doi: 10.1007/s00253-017-8657-y. Epub 2017 Nov 29.
引用本文的文献
1
Plastic pollution has the potential to alter ecological and evolutionary processes in aquatic ecosystems.塑料污染有可能改变水生生态系统中的生态和进化过程。
Nat Ecol Evol. 2025 May;9(5):762-768. doi: 10.1038/s41559-025-02678-8. Epub 2025 Apr 28.
2
Recycling and Degradation Pathways of Synthetic Textile Fibers such as Polyamide and Elastane.聚酰胺和氨纶等合成纺织纤维的回收与降解途径
Glob Chall. 2025 Mar 13;9(4):2400163. doi: 10.1002/gch2.202400163. eCollection 2025 Apr.
3
Comparative proteomics analysis of biofilms and planktonic cells of Enterococcus faecalis and Staphylococcus lugdunensis with contrasting biofilm-forming ability.对比分析具有不同生物膜形成能力的粪肠球菌和路邓葡萄球菌的生物膜和浮游细胞的比较蛋白质组学分析。
PLoS One. 2024 May 29;19(5):e0298283. doi: 10.1371/journal.pone.0298283. eCollection 2024.
4
-Caprolactam- and Nylon Oligomer-Degrading Bacterium BS3: Characterization and Potential Use in Bioremediation.-己内酰胺和尼龙低聚物降解菌BS3：特性及在生物修复中的潜在应用
Microorganisms. 2023 Feb 1;11(2):373. doi: 10.3390/microorganisms11020373.
5
Microbial enzymes will offer limited solutions to the global plastic pollution crisis.微生物酶将为全球塑料污染危机提供有限的解决方案。
Microb Biotechnol. 2023 Feb;16(2):195-217. doi: 10.1111/1751-7915.14135. Epub 2022 Sep 13.
6
Beyond nylon 6: polyamides ring opening polymerization of designer lactam monomers for biomedical applications.超越尼龙 6：用于生物医学应用的设计内酯单体的聚酰胺开环聚合。
Chem Soc Rev. 2022 Oct 3;51(19):8258-8275. doi: 10.1039/d1cs00930c.
7
Switched reaction specificity in polyesterases towards amide bond hydrolysis by enzyme engineering.通过酶工程改变聚酯酶对酰胺键水解的反应特异性。
RSC Adv. 2019 Nov 7;9(62):36217-36226. doi: 10.1039/c9ra07519d. eCollection 2019 Nov 4.
8
Isolation and genomic analysis of 11-aminoundecanoic acid-degrading bacterium sp. JG-B from nylon 11 enrichment culture.从尼龙11富集培养物中分离并对11-氨基十一烷酸降解菌JG-B进行基因组分析。
J Genomics. 2020 Jan 25;8:16-20. doi: 10.7150/jgen.42095. eCollection 2020.
9
Plastics: Environmental and Biotechnological Perspectives on Microbial Degradation.塑料：微生物降解的环境和生物技术视角。
Appl Environ Microbiol. 2019 Sep 17;85(19). doi: 10.1128/AEM.01095-19. Print 2019 Oct 1.
10
ε-Caprolactam Utilization by Proteus sp. and Bordetella sp. Isolated From Solid Waste Dumpsites in Lagos State, Nigeria, First Report.聚己内酰胺利用由尼日利亚拉各斯州固体废物堆放场分离的变形杆菌属和伯氏菌属，首次报道。
Indian J Microbiol. 2013 Jun;53(2):221-6. doi: 10.1007/s12088-013-0356-5. Epub 2013 Jan 19.
本文引用的文献
1
Characterization of the 6-aminohexanoate-dimer hydrolase from Pseudomonas sp. NK87.来自假单胞菌属NK87的6-氨基己酸二聚体水解酶的特性分析。
J Gen Microbiol. 1993 Apr;139(4):787-95. doi: 10.1099/00221287-139-4-787.
2
Recombination in adaptive mutation.适应性突变中的重组
Science. 1994 Apr 8;264(5156):258-60. doi: 10.1126/science.8146657.
3
Adaptive reversion of a frameshift mutation in Escherichia coli by simple base deletions in homopolymeric runs.通过同聚物序列中的简单碱基缺失实现大肠杆菌移码突变的适应性回复。
Science. 1994 Jul 15;265(5170):407-9. doi: 10.1126/science.8023164.
4
Adaptive mutation by deletions in small mononucleotide repeats.小单核苷酸重复序列缺失导致的适应性突变。
Science. 1994 Jul 15;265(5170):405-7. doi: 10.1126/science.8023163.
5
The directed mutation controversy and neo-Darwinism.定向突变争议与新达尔文主义。
Science. 1993 Jan 8;259(5092):188-94. doi: 10.1126/science.7678468.
6
Plasmid control of 6-aminohexanoic acid cyclic dimer degradation enzymes of Flavobacterium sp. KI72.黄杆菌属KI72菌株6-氨基己酸环状二聚体降解酶的质粒控制
J Bacteriol. 1980 Jul;143(1):238-45. doi: 10.1128/jb.143.1.238-245.1980.
7
Purification and characterization of 6-aminohexanoic-acid-oligomer hydrolase of Flavobacterium sp. Ki72.黄杆菌属Ki72菌株6-氨基己酸寡聚体水解酶的纯化与特性分析
Eur J Biochem. 1981 Jun 1;116(3):547-51. doi: 10.1111/j.1432-1033.1981.tb05371.x.
8
Evolutionary adaptation of plasmid-encoded enzymes for degrading nylon oligomers.用于降解尼龙低聚物的质粒编码酶的进化适应性
Nature. 1983;306(5939):203-6. doi: 10.1038/306203a0.
9
Plasmid-determined enzymatic degradation of nylon oligomers.质粒决定的尼龙低聚物的酶促降解。
J Bacteriol. 1983 Jul;155(1):22-31. doi: 10.1128/jb.155.1.22-31.1983.
10
Genome organization in Pseudomonas.
Annu Rev Microbiol. 1986;40:79-105. doi: 10.1146/annurev.mi.40.100186.000455.
Zotero 插件