Harpin S, Elahi S M, Cornaglia E, Yolken R H, Elazhary Y
Department of Pathology and Immunology, Faculty of Veterinary Medicine, University of Montreal, St-Hyacinthe, Quebec, Canada.
Arch Virol. 1995;140(7):1285-90. doi: 10.1007/BF01322754.
The 5' untranslated region (UTR) of several bovine viral diarrhea virus (BVDV) isolates from the severe Quebec outbreak was amplified by polymerase chain reaction (PCR) and sequenced. Sequences revealed the loss, for the BVDV type II isolates, of an internal PstI restriction site, which is present in all known BVDV type 1 5' UTR sequences. A single restriction enzyme digestion (PstI) of an aliquot of PCR product allowed us to differentiate BVDV type I and BVDV type II.
通过聚合酶链反应（PCR）扩增并测序了来自魁北克严重疫情的几种牛病毒性腹泻病毒（BVDV）分离株的5'非翻译区（UTR）。序列显示，II型BVDV分离株缺失了一个内部PstI限制性酶切位点，而该位点存在于所有已知的1型BVDV 5'UTR序列中。对PCR产物的一份等分试样进行单一限制性酶切（PstI），使我们能够区分I型BVDV和II型BVDV。
