Pandey H P, Ram A, Matsumura H, Satoh S, Hayaishi O
Osaka Bioscience Institute, Japan.
Biochem Biophys Res Commun. 1995 Aug 15;213(2):625-9. doi: 10.1006/bbrc.1995.2177.
Circadian variations in the levels of prostaglandins (PGs) D2, E2, and F2 alpha were studied in the cerebrospinal fluid (CSF) of rats. We collected CSF samples from the cisterna magna of anesthetized rats at different clock-hours (1000, 1400, 1800, 2200, 0200, and 0600 hr), and measured the concentrations of the three PGs. PGD2, which appeared to be the most abundant among the three, showed a circadian variation; and the mean of the day-time levels (145 pg/ml) was significantly higher than that of the nighttime ones (111 pg/ml). Day/night variations were also noticed with the levels of PGE2 and PGF2 alpha; however, these levels remained 3-4 times lower than those of PGD2. The general day/night variation seen in the CSF concentration of PGD2 conforms well with the postulated role of PGD2 as an endogenous sleep-promoting factor acting on a certain brain surface area defined as its site of action.
我们研究了大鼠脑脊液（CSF）中前列腺素（PGs）D2、E2和F2α水平的昼夜变化。我们在不同的时钟时间（1000、1400、1800、2200、0200和0600时）从麻醉大鼠的枕大池采集脑脊液样本，并测量这三种前列腺素的浓度。PGD2似乎是三者中含量最丰富的，呈现出昼夜变化；白天水平的平均值（145 pg/ml）显著高于夜间水平（111 pg/ml）。PGE2和PGF2α的水平也存在昼夜变化；然而，这些水平比PGD2的水平低3 - 4倍。在脑脊液中观察到的PGD2浓度的总体昼夜变化与PGD2作为内源性促睡眠因子作用于定义为其作用部位的特定脑表面积的假设作用非常吻合。
