清醒大鼠脑脊液中前列腺素D2的浓度呈现昼夜变化。

Concentration of prostaglandin D2 in cerebrospinal fluid exhibits a circadian alteration in conscious rats.

作者信息

Pandey H P, Ram A, Matsumura H, Hayaishi O

机构信息

Osaka Bioscience Institute, Furuedai, Japan.

出版信息

Biochem Mol Biol Int. 1995 Oct;37(3):431-7.

PMID:8595382

Abstract

Cerebrospinal fluid (CSF) was withdrawn from the cisterna magna of unanesthetized conscious rats (n = 14) through a chronically implanted catheter, and prostaglandins (PGs) D2, E2, and F2 alpha were measured. From each rat, CSF samples were taken at different clock-hours of the day (1000, 1400, and 1800 hr) and night (2200, 0200, and 0600 hr) in succession at 76-hour intervals. The concentration of PGD2 alone exhibited a significant circadian fluctuation, with its peak value at 1400 hr (mean +/- SEM: 1197 +/- 361 pg/ml) and its lowest level at 0600 hr (438 +/- 106 pg/ml). Thus, the mean level of PGD2 during the daytime (903 +/- 162 pg/ml) was significantly higher than that during the night (503 +/- 78 pg/ml). The results obtained are consistent with the postulated role of PGD2, acting in the surface layer of the rostral basal forebrain, as an endogenous factor to promote sleep.

摘要

通过一根长期植入的导管，从未麻醉的清醒大鼠（n = 14）的大池抽取脑脊液（CSF），并测定前列腺素（PGs）D2、E2和F2α。从每只大鼠中，在一天的不同时钟时间（1000、1400和1800时）以及夜间（2200、0200和0600时），每隔76小时连续采集脑脊液样本。仅PGD2的浓度呈现出显著的昼夜波动，其峰值出现在1400时（平均值±标准误：1197±361 pg/ml），最低水平出现在0600时（438±106 pg/ml）。因此，白天PGD2的平均水平（903±162 pg/ml）显著高于夜间（503±78 pg/ml）。所得结果与PGD2在脑前端基底前脑表层作为促进睡眠的内源性因子所假定的作用一致。