Carapetis J, Gardiner D, Currie B, Mathews J D
Menzies School of Health Research, Darwin, Australia.
J Clin Microbiol. 1995 Jun;33(6):1471-2. doi: 10.1128/jcm.33.6.1471-1472.1995.
A molecular technique (random amplification of polymorphic DNA) was used to characterize group A streptococcal (GAS) strains among 194 isolates from 55 swabs from 12 Australian Aboriginal children and adults with multiple pyoderma lesions. Ninety-three percent of the lesions contained only one strain of GAS, but 8 of 12 individuals were infected with more than one strain. We conclude that accurate epidemiologic surveys require that more than one swab specimen be obtained from each person, whereas typing of more than one colony per swab is less informative. Characterization of GAS strains by random amplification of polymorphic DNA analysis should help to provide important insights into the epidemiology of GAS, particularly in tropical populations where many isolates are M nontypeable, and into the mechanisms of genetic variation of GAS in such populations.
采用一种分子技术（多态性DNA随机扩增）对来自12名患有多发性脓皮病皮损的澳大利亚原住民儿童和成人的55份拭子样本中的194株A组链球菌（GAS）菌株进行特征分析。93%的皮损仅含有一株GAS，但12名个体中有8名感染了不止一株菌株。我们得出结论，准确的流行病学调查要求从每个人身上获取不止一份拭子样本，而对每份拭子上的多个菌落进行分型则信息量较少。通过多态性DNA分析随机扩增对GAS菌株进行特征分析，应有助于深入了解GAS的流行病学，特别是在许多分离株无法进行M分型的热带人群中，以及了解此类人群中GAS的遗传变异机制。
