Arribas J R, Clifford D B, Fichtenbaum C J, Roberts R L, Powderly W G, Storch G A
Department of Medicine, Washington University School of Medicine, St. Louis, Missouri 63138, USA.
J Clin Microbiol. 1995 Jun;33(6):1580-3. doi: 10.1128/jcm.33.6.1580-1583.1995.
The diagnostic utility of Epstein-Barr virus (EBV) DNA detection in cerebrospinal fluid for the diagnosis of central nervous system lymphoma was evaluated with two different PCR assays to test a collection of cerebrospinal fluid samples from 24 AIDS patients with central nervous system disorders. A PCR assay amplifying a fragment from the BamHI-W region had the highest clinical and analytic sensitivity. The BamHI-W PCR assay detected EBV DNA in cerebrospinal fluid from 83% (5 of 6) of patients with pathologically proven primary central nervous system lymphoma and 7% (1 of 16) of controls with autopsy-proven nonlymphomatous central nervous system disorders. EBV DNA was also detected in one patient with autopsy-proven systemic lymphoma involving the central nervous system and one patient with probable primary central nervous system lymphoma. EBV DNA was detected consistently when central nervous system lymphoma involved meningeal surfaces. PCR for EBV in cerebrospinal fluid appears to be useful for diagnosis of AIDS-related central nervous system lymphoma, but additional studies are required to better define the sensitivity of the assay and to understand the significance of a positive test in the absence of lymphoma.
采用两种不同的聚合酶链反应（PCR）检测法，对24例患有中枢神经系统疾病的艾滋病患者的脑脊液样本进行检测，评估脑脊液中爱泼斯坦-巴尔病毒（EBV）DNA检测对中枢神经系统淋巴瘤诊断的效用。一种扩增BamHI-W区域片段的PCR检测法具有最高的临床和分析灵敏度。BamHI-W PCR检测法在6例经病理证实的原发性中枢神经系统淋巴瘤患者中的83%（5/6）的脑脊液中检测到EBV DNA，在16例经尸检证实为非淋巴瘤性中枢神经系统疾病的对照患者中的7%（1/16）检测到EBV DNA。在1例经尸检证实有中枢神经系统受累的系统性淋巴瘤患者和1例可能患有原发性中枢神经系统淋巴瘤的患者中也检测到了EBV DNA。当中枢神经系统淋巴瘤累及脑膜表面时，可始终检测到EBV DNA。脑脊液中EBV的PCR检测似乎有助于艾滋病相关中枢神经系统淋巴瘤的诊断，但需要进一步研究以更好地确定该检测法的灵敏度，并了解在无淋巴瘤情况下阳性检测结果的意义。
