南非丙型肝炎病毒的基因分型
Genotyping of hepatitis C virus in South Africa.
作者信息
Smuts H E, Kannemeyer J
机构信息
Department of Medical Microbiology, University of Cape Town Medical School, Observatory, South Africa.
出版信息
J Clin Microbiol. 1995 Jun;33(6):1679-81. doi: 10.1128/jcm.33.6.1679-1681.1995.
Abstract
The six major hepatitis C virus genotypes were investigated by using samples from 79 seropositive and PCR-positive blood donors from three different regions of South Africa as well as 9 patients with chronic renal failure, 19 with liver disease, and 23 with hemophilia. PCR products of the genome were typed by restriction fragment length polymorphic analysis by RsaI-HaeIII and MvaI-HinfI double digestion. Type 5 occurred in 40% of this population group; type 1 occurred in 33%; and types 2, 3, and 4 were found in 13.8, 7.7, and 2.3%, respectively.
摘要
通过使用来自南非三个不同地区的79名血清学阳性且PCR阳性的献血者的样本，以及9名慢性肾衰竭患者、19名肝病患者和23名血友病患者的样本，对六种主要丙型肝炎病毒基因型进行了研究。通过RsaI-HaeIII和MvaI-HinfI双酶切的限制性片段长度多态性分析对基因组的PCR产物进行分型。该人群组中5型占40%；1型占33%；2型、3型和4型分别占13.8%、7.7%和2.3%。
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