Romanos G E, Pelekanos S, Strub J R
Department of Prosthodontics, Dental School Freiburg, Albert-Ludwigs-University, Germany.
Lasers Surg Med. 1995;16(4):368-79. doi: 10.1002/lsm.1900160408.
The clinical effects of the Nd:YAG laser in the rat skin as well as alterations of the extracellular matrix during healing were presented in this study.
STUDY DESIGN/MATERIAL AND METHODS: This study evaluated the clinical effects of the Nd:YAG laser used with different energy parameters (low energy: 1.75 W and 20 pps/high energy: 3.0 W and 30 pps) in a duration 20-40 s in rat skin. Control incisions were performed with a scalpel blade. Rat skin incisions were examined over a period of 28 days by clinical photographs as well as by using immunohistochemical techniques in order to find the distribution and the amount of the extracellular matrix fibrillar components, i.e., collagen types I and III.
Low energy laser treatment caused a rapid wound healing without scar tissue formation (compared to the high energy laser group) and clinical signs of scar tissue formation (compared to control incisions with the conventional scalpel). During the study period, the laser-induced lesions healed through reparative synthesis of the matrix proteins, which led to filling of the tissue defects. The regenerative processes were similar in the low-energy laser group and in the control incisions. In the high-energy lased tissues, we observed a delayed replacement of the defects by newly formed extracellular matrix proteins.
This study showed a slower wound healing in the high-energy lased tissues. A similar healing in the low-energy lased tissues and in the incisions with the conventional scalpel was observed. The differences in the distribution of matrix proteins during healing and the coagulation of the tissues, which were exposed to low-energy laser treatment, might be the explanation for the minimal scarring, contraction, and pigmentation of the lased tissues as compared to conventional incisions.
本研究展示了钕钇铝石榴石激光对大鼠皮肤的临床效果以及愈合过程中细胞外基质的变化。
研究设计/材料与方法：本研究评估了不同能量参数（低能量：1.75瓦和20脉冲每秒/高能量：3.0瓦和30脉冲每秒）的钕钇铝石榴石激光在大鼠皮肤上持续20 - 40秒的临床效果。对照切口用手术刀刀片进行。通过临床照片以及免疫组织化学技术对大鼠皮肤切口进行28天的观察，以确定细胞外基质纤维成分即I型和III型胶原蛋白的分布和数量。
低能量激光治疗导致伤口快速愈合且无瘢痕组织形成（与高能量激光组相比）以及无瘢痕组织形成的临床迹象（与传统手术刀对照切口相比）。在研究期间，激光诱导的损伤通过基质蛋白的修复性合成愈合，这导致组织缺损的填充。低能量激光组和对照切口中的再生过程相似。在高能量激光照射的组织中，我们观察到新形成的细胞外基质蛋白对缺损的替代延迟。
本研究表明高能量激光照射的组织伤口愈合较慢。观察到低能量激光照射的组织和传统手术刀切口的愈合情况相似。与传统切口相比，愈合过程中基质蛋白分布的差异以及低能量激光治疗组织的凝固现象，可能是激光照射组织瘢痕形成、收缩和色素沉着最小化的原因。
