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从小鼠大脑中分离出的单链DNA结合蛋白在体外能识别特定的三核苷酸重复序列。

Single-stranded DNA binding proteins isolated from mouse brain recognize specific trinucleotide repeat sequences in vitro.

作者信息

Yano-Yanagisawa H, Li Y, Wang H, Kohwi Y

机构信息

La Jolla Cancer Research Foundation, CA 92037, USA.

出版信息

Nucleic Acids Res. 1995 Jul 25;23(14):2654-60. doi: 10.1093/nar/23.14.2654.

DOI:10.1093/nar/23.14.2654
PMID:7651826
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC307089/
Abstract

Expansion of trinucleotide repeats (CAG)n and (CGG)n is found in genes responsible for certain human hereditary neurodegenerative diseases. By gel-mobility shift assay, we detected a single-stranded (AGC)n repeat-binding activity primarily in mouse brain extracts and very low or undetectable activity in other tissue extracts. Two (AGC)n-repeat binding proteins, with apparent molecular weights of 44 and 40 kDa, have been purified from mouse adult brain by a DNA affinity column and fast protein liquid chromatography. UV-cross linking of radiolabeled (AGC)n repeats with crude brain extracts and with purified two proteins of 44 and 40 kDa produced identical doublet bands, indicating that these proteins are in fact responsible for the (AGC)n-binding activity in brain extracts. We designated these two proteins TRIP-1 for the 44 kDa protein and TRIP-2 for the 40 kDa protein, where TRIP represents trinucleotide repeat-binding protein. TRIP-1 and TRIP-2 bind to a specific subset of trinucleotide repeat sequences including (AGC)n, (AGT)n, (GGC)n, and (GGT)n repeats but not to various other trinucleotide repeats. A minimum of eight (AGC) trinucleotide repeating units is required for TRIP-1 and -2 recognition and binding. The (AGC)n repeat-binding activity increases in the brain after birth and reaches a plateau within 3 weeks. In the brain, TRIP-1 and TRIP-2 may alter the function of the genes containing the expanded-trinucleotide repeats.

摘要

在某些人类遗传性神经退行性疾病相关基因中发现了三核苷酸重复序列（CAG）n和（CGG）n的扩增。通过凝胶迁移率变动分析，我们检测到单链（AGC）n重复序列结合活性，主要存在于小鼠脑提取物中，而在其他组织提取物中活性很低或无法检测到。通过DNA亲和柱和快速蛋白质液相色谱法，从成年小鼠脑中纯化出了两种（AGC）n重复序列结合蛋白，其表观分子量分别为44 kDa和40 kDa。用放射性标记的（AGC）n重复序列与脑粗提物以及纯化的44 kDa和40 kDa的两种蛋白质进行紫外线交联，产生了相同的双峰带，表明这些蛋白质实际上负责脑提取物中的（AGC）n结合活性。我们将这两种蛋白质分别命名为TRIP - 1（44 kDa蛋白质）和TRIP - 2（40 kDa蛋白质），其中TRIP代表三核苷酸重复序列结合蛋白。TRIP - 1和TRIP - 2与三核苷酸重复序列的特定子集结合，包括（AGC）n、（AGT）n、（GGC）n和（GGT）n重复序列，但不与其他各种三核苷酸重复序列结合。TRIP - 1和 - 2识别和结合至少需要八个（AGC）三核苷酸重复单元。出生后，脑中的（AGC）n重复序列结合活性增加，并在3周内达到稳定水平。在脑中，TRIP - 1和TRIP - 2可能会改变含有扩增三核苷酸重复序列的基因的功能。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b61/307089/787ece950878/nar00014-0097-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b61/307089/5e7be7befdce/nar00014-0094-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b61/307089/000b64535ef6/nar00014-0094-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b61/307089/676269e5837f/nar00014-0095-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b61/307089/de44eeccedb8/nar00014-0096-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b61/307089/11130caad5c5/nar00014-0096-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b61/307089/17cc5661bc98/nar00014-0096-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b61/307089/787ece950878/nar00014-0097-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b61/307089/5e7be7befdce/nar00014-0094-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b61/307089/000b64535ef6/nar00014-0094-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b61/307089/676269e5837f/nar00014-0095-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b61/307089/de44eeccedb8/nar00014-0096-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b61/307089/11130caad5c5/nar00014-0096-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b61/307089/17cc5661bc98/nar00014-0096-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b61/307089/787ece950878/nar00014-0097-a.jpg

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本文引用的文献

1
Origin of the expansion mutation in myotonic dystrophy.强直性肌营养不良症中扩增突变的起源。
Nat Genet. 1993 May;4(1):72-6. doi: 10.1038/ng0593-72.
2
The relationship between trinucleotide (CAG) repeat length and clinical features of Huntington's disease.三核苷酸（CAG）重复序列长度与亨廷顿舞蹈症临床特征之间的关系。
Nat Genet. 1993 Aug;4(4):398-403. doi: 10.1038/ng0893-398.
3
Relationship between trinucleotide repeat expansion and phenotypic variation in Huntington's disease.亨廷顿舞蹈病中三核苷酸重复序列扩增与表型变异的关系。
Mol Cell Biol. 1997 Apr;17(4):2090-8. doi: 10.1128/MCB.17.4.2090.
Nat Genet. 1993 Aug;4(4):393-7. doi: 10.1038/ng0893-393.
4
Trinucleotide repeat length instability and age of onset in Huntington's disease.亨廷顿病中三核苷酸重复序列长度不稳定性与发病年龄
Nat Genet. 1993 Aug;4(4):387-92. doi: 10.1038/ng0893-387.
5
Transcription-dependent recombination induced by triple-helix formation.三链螺旋形成诱导的转录依赖性重组。
Genes Dev. 1993 Sep;7(9):1766-78. doi: 10.1101/gad.7.9.1766.
6
Expansion of an unstable trinucleotide CAG repeat in spinocerebellar ataxia type 1.1型脊髓小脑共济失调中不稳定的三核苷酸CAG重复序列的扩增。
Nat Genet. 1993 Jul;4(3):221-6. doi: 10.1038/ng0793-221.
7
Trinucleotide repeat amplification and hypermethylation of a CpG island in FRAXE mental retardation.脆性X染色体E型智力障碍中三核苷酸重复扩增及一个CpG岛的高甲基化
Cell. 1993 Jul 16;74(1):127-34. doi: 10.1016/0092-8674(93)90300-f.
8
Nuclear proteins that bind the pre-mRNA 3' splice site sequence r(UUAG/G) and the human telomeric DNA sequence d(TTAGGG)n.与前体mRNA 3'剪接位点序列r(UUAG/G)和人类端粒DNA序列d(TTAGGG)n结合的核蛋白。
Mol Cell Biol. 1993 Jul;13(7):4301-10. doi: 10.1128/mcb.13.7.4301-4310.1993.
9
A simple method to renature DNA-binding proteins separated by SDS-polyacrylamide gel electrophoresis.一种使经十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分离的DNA结合蛋白复性的简单方法。
Nucleic Acids Res. 1993 Dec 25;21(25):6040-1. doi: 10.1093/nar/21.25.6040.
10
A single trinucleotide, 5'AGC3'/5'GCT3', of the triplet-repeat disease genes confers metal ion-induced non-B DNA structure.三联体重复疾病基因中的单个三核苷酸5'AGC3'/5'GCT3'可导致金属离子诱导的非B型DNA结构。
Nucleic Acids Res. 1993 Dec 11;21(24):5651-5. doi: 10.1093/nar/21.24.5651.