Sugiyama K, Ueda H, Ichio Y
School of Pharmaceutical Sciences, University of Shizuoka, Japan.
Biol Pharm Bull. 1995 Apr;18(4):544-8. doi: 10.1248/bpb.18.544.
The effect of an oral treatment with the Kampo formulation Juzen-taiho-to on the toxicity caused by the intraperitoneal administration of 15 mg/kg carboplatin (CBDCA) 9 times (on days 3, 4, 5, 6, 7, 8, 10, 11 and 12) was examined in ddY mice, which were subcutaneously inoculated with sarcoma 180 (S-180) cells on day 1. White blood cell counts, platelet counts, bone marrow cell counts, relative spleen and thymus weight, food intake and body weight decreased significantly, to about 29%, 13%, 14%, 59%, 36%, 42% and 72% of the control levels, respectively, and serum glutamic-oxaloacetatic transaminase, serum glutamic-pyruvic transaminase and relative stomach weight increased significantly, to about 4, 6 and 3 times the control levels, respectively, by the treatment with CBDCA. However, the blood urea nitrogen and serum creatinine were only slightly increased compared to the control value. Co-treatment with 1.7 g/kg of a lyophilized water extract of Juzen-taiho-to once a day 12 times (on days 3, 4, 5, 6, 7, 8, 10, 11, 12, 13, 14 and 15) prevented both the decreases and increases caused by CBDCA to near the control levels without reducing the antitumor activity of CBDCA against S-180. The inhibitory effect of Juzen-taiho-to against CBDCA-induced myelosuppression was similar to that against 3.0 mg/kg cisplatin (CDDP) 9 times, while CBDCA-induced myelosuppression was more serious in comparison with CDDP. Therefore, these findings indicate that Juzen-taiho-to could be an effective drug for protecting against the side effects induced by CBDCA in the clinic as well as by CDDP.
在第1天皮下接种肉瘤180（S - 180）细胞的ddY小鼠中，研究了口服汉方制剂十全大补汤对腹腔注射15mg/kg卡铂（CBDCA）9次（第3、4、5、6、7、8、10、11和12天）所引起毒性的影响。卡铂治疗使白细胞计数、血小板计数、骨髓细胞计数、脾脏和胸腺相对重量、食物摄入量及体重显著下降，分别降至对照水平的约29%、13%、14%、59%、36%、42%和72%，同时血清谷草转氨酶、血清谷丙转氨酶及胃相对重量显著升高，分别升至对照水平的约4倍、6倍和3倍。然而，血尿素氮和血清肌酐与对照值相比仅略有升高。十全大补汤冻干粉水提取物1.7g/kg每日一次共12次（第3、4、5、6、7、8、10、11、12、13、14和15天）联合治疗可防止卡铂引起的下降和升高，使其接近对照水平，且不降低卡铂对S - 180的抗肿瘤活性。十全大补汤对卡铂诱导的骨髓抑制的抑制作用与对9次注射3.0mg/kg顺铂（CDDP）的抑制作用相似，而卡铂诱导的骨髓抑制比顺铂更严重。因此，这些结果表明，十全大补汤在临床上可能是一种有效药物，可预防卡铂以及顺铂诱导的副作用。
