Beck S C, Paidas C N, Mooney M L, Deitch E A, De Maio A
Division of Pediatric Surgery, Johns Hopkins University School of Medicine, Baltimore, Maryland 21287, USA.
Shock. 1995 Jun;3(6):398-402.
The expression of heat shock proteins (hsp) is probably one of the most primitive mechanisms of cellular protection from stress. Pathogens such as viruses and bacteria have recently been found to induce the heat shock gene expression. In the present study hsp-72, the stress-inducible form of hsp-70, was detected by Western blotting in samples from rat distal colon (DC), proximal colon (PC), and terminal ileum (TI), but was not found in proximal small bowel (PSB) or other organs (liver, kidney, spleen, heart, and brain) of unstressed animals. The signal intensity of hsp-72 in colon (DC > PC > TI > PSB) correlates qualitatively with the presence of normal gut microflora. hsp-72 was also observed in DC, to a lesser extent in PC, but not in TI or PSB of bacteria-free or antibiotic-treated rats. Inflammatory states induced by the intravenous administration of endotoxin (1 mg/kg), the subcutaneous injection of zymosan (1 g/kg) or by cecal ligation and puncture (sepsis) failed to increase the hsp-72 levels in rat colon or other organs. These results demonstrate that hsp-72 is expressed in normal rat colon. However, the induction of hsp-72 expression may not be due solely to the presence of resident bacteria in the gut, but instead, may be the result of a more complex process.
热休克蛋白（hsp）的表达可能是细胞抵御应激最原始的机制之一。最近发现，病毒和细菌等病原体可诱导热休克基因表达。在本研究中，通过蛋白质免疫印迹法在大鼠远端结肠（DC）、近端结肠（PC）和回肠末端（TI）的样本中检测到hsp-70的应激诱导形式hsp-72，但在未受应激动物的近端小肠（PSB）或其他器官（肝脏、肾脏、脾脏、心脏和大脑）中未发现。结肠中hsp-72的信号强度（DC > PC > TI > PSB）与正常肠道微生物群的存在在质量上相关。在无菌或经抗生素处理的大鼠的DC中也观察到hsp-72，在PC中程度较轻，但在TI或PSB中未观察到。静脉注射内毒素（1 mg/kg）、皮下注射酵母聚糖（1 g/kg）或盲肠结扎和穿刺（脓毒症）诱导的炎症状态未能增加大鼠结肠或其他器官中hsp-72的水平。这些结果表明，hsp-72在正常大鼠结肠中表达。然而，hsp-72表达的诱导可能不仅仅归因于肠道中常驻细菌的存在，而是可能是一个更复杂过程的结果。
