Kissmeyer-Nielsen P, Mortensen F V, Laurberg S, Hessov I
Department of Surgery L, Amtssygehuset, University Hospital of Aarhus, Denmark.
Dis Colon Rectum. 1995 Sep;38(9):946-51. doi: 10.1007/BF02049730.
This study was designed to investigate and quantify trophic alterations in the defunctioned, atrophic rat colon after short chain fatty acid (SCFA) treatment was administered in a clinically relevant way.
Diverting colostomy with exclusion of distal colon was performed on adult female rats (58), and treatment was started four weeks later. Enemas of either a SCFA solution of sodium-acetate, sodium-propionate and sodium-butyrate (concentration, 780 mM), or isotonic saline (placebo) were instilled through the anus into the defunctioned colon. This was done twice daily for 7 or 14 days before death.
After SCFA instillation for 14 days, the colonic wet weight was 18 percent higher compared with placebo (P < 0.01), and there was a similarly significant difference in dry weight (P < 0.05). Using stereologic assessment to determine the histologic composition of defunctioned colon, we found significant increases among SCFA-treated rats in the weight of the mucose (P < 0.05), the submucosa (P < 0.05), and the muscularis propria (P < 0.05) and a 30 percent increase in the mucosal surface area compared with placebo-treated in the mucosal surface area compared with placebo-treated rats (P < 0.05). Measurements of breaking strength and hydroxyproline content showed no differences between treatment groups.
SCFA enemas have a transmural trophic effect and preserve mucosal surface area of defunctioned and atrophic colon in rats.
本研究旨在以临床相关方式给予短链脂肪酸（SCFA）治疗后，调查并量化功能丧失的萎缩性大鼠结肠中的营养改变。
对成年雌性大鼠（58只）施行远端结肠旷置的转流性结肠造口术，4周后开始治疗。通过肛门将醋酸钠、丙酸钠和丁酸钠的SCFA溶液（浓度780 mM）或等渗盐水（安慰剂）灌肠至功能丧失的结肠。在处死前，每天进行2次，持续7天或14天。
SCFA灌注14天后，结肠湿重比安慰剂组高18%（P < 0.01），干重也有类似的显著差异（P < 0.05）。使用体视学评估来确定功能丧失结肠的组织学组成，我们发现SCFA治疗的大鼠中，黏膜（P < 0.05）、黏膜下层（P < 0.05）和固有肌层（P < 0.05）的重量显著增加，与安慰剂治疗的大鼠相比，黏膜表面积增加了30%（P < 0.05）。抗张强度和羟脯氨酸含量的测量显示治疗组之间没有差异。
SCFA灌肠对大鼠功能丧失和萎缩的结肠具有透壁营养作用，并能保留其黏膜表面积。
