Ricard-Blum S, Chevalier X, Grimaud J A, Larget-Piet B, Uebelhart D
Institut Pasteur de Lyon, France.
Eur J Clin Invest. 1995 Jun;25(6):438-41. doi: 10.1111/j.1365-2362.1995.tb01726.x.
There is a major interest for using biochemical markers of bone metabolism as a non-invasive tool for diagnostic purposes in the field of bone and joint diseases. Based upon the fact that the pyridinium cross-links of collagen are markers of bone and cartilage degradation, this study was designed to assess the presence of pyridinoline in synovial fluid samples originating from various arthritic and non-arthritic knee joints. Using a sample pooling method, significant levels of pyridinoline could be measured in synovial fluid by high performance liquid chromatography. Pyridinoline levels ranged from 19.3 +/- 5.8 pmol mL-1 (mean +/- SD) in osteoarthritic knee joints up to 32.4 +/- 14.6 pmol mL-1 in rheumatoid arthritis joints. Pyridinoline levels in synovial fluid were not significantly correlated to disease duration and synovial fluid cell count, but were correlated to erythrocyte sedimentation rate in osteoarthritic patients (r = 0.99, P = 0.002). This study demonstrates that synovial fluid originating from knee effusion contains significant levels of pyridinoline which can be quantified by high performance liquid chromatography and could, therefore, be a tool to investigate the metabolism of a single joint.
在骨与关节疾病领域，将骨代谢生化标志物用作非侵入性诊断工具具有重大意义。基于胶原蛋白的吡啶交联物是骨和软骨降解标志物这一事实，本研究旨在评估来自各种关节炎性和非关节炎性膝关节的滑液样本中吡啶啉的存在情况。采用样本合并方法，通过高效液相色谱法可在滑液中检测到显著水平的吡啶啉。吡啶啉水平在骨关节炎膝关节中为19.3±5.8 pmol/mL（平均值±标准差），在类风湿关节炎关节中高达32.4±14.6 pmol/mL。滑液中的吡啶啉水平与病程和滑液细胞计数无显著相关性，但与骨关节炎患者的红细胞沉降率相关（r = 0.99，P = 0.002）。本研究表明，源自膝关节积液的滑液含有显著水平的吡啶啉，可通过高效液相色谱法定量，因此可能是研究单个关节代谢的一种工具。
