pto细菌抗性基因和Fen杀虫剂敏感性基因编码具有丝氨酸/苏氨酸特异性的功能性蛋白激酶。
The Pto bacterial resistance gene and the Fen insecticide sensitivity gene encode functional protein kinases with serine/threonine specificity.
作者信息
Loh Y T, Martin G B
机构信息
Department of Agronomy, Purdue University, West Lafayette, Indiana 47907-1150, USA.
出版信息
Plant Physiol. 1995 Aug;108(4):1735-9. doi: 10.1104/pp.108.4.1735.
Abstract
The catalytic activity and amino acid specificity of the tomato Pto and Fen kinases were investigated. The Pto and Fen genes were fused to the carboxyl terminus of the maltose-binding protein and expressed in Escherichia coli. Incubation of the purified fusion proteins with [gamma-32P]ATP in an in vitro assay showed that both proteins were capable of autophosphorylation. Mutant fusion proteins in which the conserved lysine residue of subdomain II was changed to a glutamine were unable to autophosphorylate. Phosphoamino analysis of the active fusion proteins indicated that both kinases phosphorylate serine and threonine residues but not tyrosine.
摘要
对番茄Pto和Fen激酶的催化活性及氨基酸特异性进行了研究。将Pto和Fen基因融合至麦芽糖结合蛋白的羧基末端，并在大肠杆菌中表达。在体外试验中，将纯化的融合蛋白与[γ-32P]ATP一起温育，结果表明这两种蛋白都能够进行自身磷酸化。亚结构域II中保守赖氨酸残基被替换为谷氨酰胺的突变融合蛋白无法进行自身磷酸化。对活性融合蛋白的磷酸氨基酸分析表明，这两种激酶都能磷酸化丝氨酸和苏氨酸残基，但不能磷酸化酪氨酸残基。
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The Pto bacterial resistance gene and the Fen insecticide sensitivity gene encode functional protein kinases with serine/threonine specificity.pto细菌抗性基因和Fen杀虫剂敏感性基因编码具有丝氨酸/苏氨酸特异性的功能性蛋白激酶。
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