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1
The Pto bacterial resistance gene and the Fen insecticide sensitivity gene encode functional protein kinases with serine/threonine specificity.pto细菌抗性基因和Fen杀虫剂敏感性基因编码具有丝氨酸/苏氨酸特异性的功能性蛋白激酶。
Plant Physiol. 1995 Aug;108(4):1735-9. doi: 10.1104/pp.108.4.1735.
2
The disease-resistance gene Pto and the fenthion-sensitivity gene fen encode closely related functional protein kinases.抗病基因Pto和倍硫磷敏感性基因fen编码密切相关的功能性蛋白激酶。
Proc Natl Acad Sci U S A. 1995 May 9;92(10):4181-4. doi: 10.1073/pnas.92.10.4181.
3
Alleles of Pto and Fen occur in bacterial speck-susceptible and fenthion-insensitive tomato cultivars and encode active protein kinases.Pto和Fen的等位基因存在于对细菌性斑点病敏感和对倍硫磷不敏感的番茄品种中，并编码活性蛋白激酶。
Plant Cell. 1997 Jan;9(1):61-73. doi: 10.1105/tpc.9.1.61.
4
A member of the tomato Pto gene family confers sensitivity to fenthion resulting in rapid cell death.番茄Pto基因家族的一个成员赋予对倍硫磷的敏感性，导致细胞迅速死亡。
Plant Cell. 1994 Nov;6(11):1543-52. doi: 10.1105/tpc.6.11.1543.
5
Use of a gene expression system based on potato virus X to rapidly identify and characterize a tomato Pto homolog that controls fenthion sensitivity.利用基于马铃薯X病毒的基因表达系统快速鉴定和表征一个控制倍硫磷敏感性的番茄Pto同源基因。
Plant Cell. 1995 Mar;7(3):249-57. doi: 10.1105/tpc.7.3.249.
6
Functional analyses of the Pto resistance gene family in tomato and the identification of a minor resistance determinant in a susceptible haplotype.番茄中Pto抗性基因家族的功能分析以及易感单倍型中一个次要抗性决定因素的鉴定。
Mol Plant Microbe Interact. 2002 Mar;15(3):281-91. doi: 10.1094/MPMI.2002.15.3.281.
7
The tomato gene Pti1 encodes a serine/threonine kinase that is phosphorylated by Pto and is involved in the hypersensitive response.番茄基因Pti1编码一种丝氨酸/苏氨酸激酶，该激酶被Pto磷酸化并参与过敏反应。
Cell. 1995 Dec 15;83(6):925-35. doi: 10.1016/0092-8674(95)90208-2.
8
The major site of the pti1 kinase phosphorylated by the pto kinase is located in the activation domain and is required for pto-pti1 physical interaction.被pto激酶磷酸化的pti1激酶的主要位点位于激活结构域，是pto与pti1物理相互作用所必需的。
Eur J Biochem. 2000 Jan;267(1):171-8. doi: 10.1046/j.1432-1327.2000.00979.x.
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Two expressed soybean genes with high sequence identity to tomato Pti1 kinase lack autophosphorylation activity.
Arch Biochem Biophys. 2000 Nov 15;383(2):233-7. doi: 10.1006/abbi.2000.2080.
10
Tomato Prf is a member of the leucine-rich repeat class of plant disease resistance genes and lies embedded within the Pto kinase gene cluster.番茄Prf是富含亮氨酸重复序列类植物抗病基因的成员，位于Pto激酶基因簇内。
Cell. 1996 Jul 12;86(1):123-33. doi: 10.1016/s0092-8674(00)80083-5.

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A diverse panel of 755 bread wheat accessions harbors untapped genetic diversity in landraces and reveals novel genetic regions conferring powdery mildew resistance.一个由 755 份小麦品种组成的多样化小组蕴藏着传统品种中未被开发的遗传多样性，并揭示了赋予小麦抗白粉病的新的遗传区域。
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Screening for potential nuclear substrates for the plant cell death suppressor kinase Adi3 using peptide microarrays.利用肽微阵列筛选植物细胞死亡抑制激酶 Adi3 的潜在核底物。
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Genome-Wide Analysis and Evolution of the Pto-Like Protein Kinase (PLPK) Gene Family in Pepper.辣椒中类Pto蛋白激酶（PLPK）基因家族的全基因组分析与进化
PLoS One. 2016 Aug 18;11(8):e0161545. doi: 10.1371/journal.pone.0161545. eCollection 2016.
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Pto kinase binds two domains of AvrPtoB and its proximity to the effector E3 ligase determines if it evades degradation and activates plant immunity.Pto激酶结合AvrPtoB的两个结构域，其与效应子E3连接酶的接近程度决定了它是否能逃避降解并激活植物免疫。
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Post-translational modification of host proteins in pathogen-triggered defence signalling in plants.植物病原体触发防御信号传导中宿主蛋白的翻译后修饰
Mol Plant Pathol. 2008 Jul;9(4):545-60. doi: 10.1111/j.1364-3703.2008.00468.x.
7
Genetic diversity of Pto-like serine/threonine kinase disease resistance genes in cultivated and wild strawberries.栽培草莓和野生草莓中类Pto丝氨酸/苏氨酸激酶抗病基因的遗传多样性
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8
The PTI1-like kinase ZmPti1a from maize (Zea mays L.) co-localizes with callose at the plasma membrane of pollen and facilitates a competitive advantage to the male gametophyte.来自玉米（Zea mays L.）的类PTI1激酶ZmPti1a与花粉质膜上的胼胝质共定位，并赋予雄配子体竞争优势。
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Subcellular localization and functions of the barley stem rust resistance receptor-like serine/threonine-specific protein kinase Rpg1.大麦抗秆锈病受体样丝氨酸/苏氨酸特异性蛋白激酶Rpg1的亚细胞定位及功能
Proc Natl Acad Sci U S A. 2006 May 9;103(19):7518-23. doi: 10.1073/pnas.0602379103. Epub 2006 Apr 28.
10
Adi3 is a Pdk1-interacting AGC kinase that negatively regulates plant cell death.Adi3是一种与Pdk1相互作用的AGC激酶，它对植物细胞死亡起负调控作用。
EMBO J. 2006 Jan 11;25(1):255-65. doi: 10.1038/sj.emboj.7600910. Epub 2005 Dec 15.

本文引用的文献

1
An Arabidopsis thaliana Gene with Sequence Similarity to the S-Locus Receptor Kinase of Brassica oleracea: Sequence and Expression.拟南芥与甘蓝型油菜 S 基因座受体激酶具有序列相似性的基因：序列和表达。
Plant Physiol. 1992 May;99(1):284-90. doi: 10.1104/pp.99.1.284.
2
cDNA Sequence of PsPK5, a Protein Kinase Homolog from Pisum sativum L.来自豌豆（Pisum sativum L.）的蛋白激酶同源物PsPK5的cDNA序列
Plant Physiol. 1992 Oct;100(2):1072-4. doi: 10.1104/pp.100.2.1072.
3
CTR1, a negative regulator of the ethylene response pathway in Arabidopsis, encodes a member of the raf family of protein kinases.CTR1是拟南芥乙烯反应途径的负调控因子，编码raf蛋白激酶家族的一个成员。
Cell. 1993 Feb 12;72(3):427-41. doi: 10.1016/0092-8674(93)90119-b.
4
A plant receptor-like gene, the S-locus receptor kinase of Brassica oleracea L., encodes a functional serine/threonine kinase.一种植物类受体基因，即甘蓝的S位点受体激酶，编码一种功能性丝氨酸/苏氨酸激酶。
Plant Physiol. 1993 Mar;101(3):1103-6. doi: 10.1104/pp.101.3.1103.
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Receptor-like protein kinase genes of Arabidopsis thaliana.拟南芥的类受体蛋白激酶基因。
Plant J. 1993 Mar;3(3):451-6. doi: 10.1111/j.1365-313x.1993.tb00164.x.
6
Arabidopsis ethylene-response gene ETR1: similarity of product to two-component regulators.拟南芥乙烯反应基因ETR1：其产物与双组分调节因子的相似性
Science. 1993 Oct 22;262(5133):539-44. doi: 10.1126/science.8211181.
7
Biochemical properties of the autophosphorylation of RLK5, a receptor-like protein kinase from Arabidopsis thaliana.来自拟南芥的类受体蛋白激酶RLK5自磷酸化的生化特性。
Biochim Biophys Acta. 1994 Sep 21;1208(1):65-74. doi: 10.1016/0167-4838(94)90160-0.
8
Characterization of a pollen-expressed receptor-like kinase gene of Petunia inflata and the activity of its encoded kinase.矮牵牛花粉表达的类受体激酶基因的特性及其编码激酶的活性
Plant Cell. 1994 May;6(5):709-21. doi: 10.1105/tpc.6.5.709.
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Molecular cloning and biochemical characterization of a receptor-like serine/threonine kinase from rice.水稻中一种类受体丝氨酸/苏氨酸激酶的分子克隆与生化特性分析
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10
Map-based cloning of a protein kinase gene conferring disease resistance in tomato.基于图谱克隆赋予番茄抗病性的蛋白激酶基因。
Science. 1993 Nov 26;262(5138):1432-6. doi: 10.1126/science.7902614.

pto细菌抗性基因和Fen杀虫剂敏感性基因编码具有丝氨酸/苏氨酸特异性的功能性蛋白激酶。

The Pto bacterial resistance gene and the Fen insecticide sensitivity gene encode functional protein kinases with serine/threonine specificity.

作者信息

Loh Y T, Martin G B

机构信息

Department of Agronomy, Purdue University, West Lafayette, Indiana 47907-1150, USA.

出版信息

Plant Physiol. 1995 Aug;108(4):1735-9. doi: 10.1104/pp.108.4.1735.

DOI:10.1104/pp.108.4.1735
PMID:7659757
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC157555/
Abstract

The catalytic activity and amino acid specificity of the tomato Pto and Fen kinases were investigated. The Pto and Fen genes were fused to the carboxyl terminus of the maltose-binding protein and expressed in Escherichia coli. Incubation of the purified fusion proteins with [gamma-32P]ATP in an in vitro assay showed that both proteins were capable of autophosphorylation. Mutant fusion proteins in which the conserved lysine residue of subdomain II was changed to a glutamine were unable to autophosphorylate. Phosphoamino analysis of the active fusion proteins indicated that both kinases phosphorylate serine and threonine residues but not tyrosine.

摘要

对番茄Pto和Fen激酶的催化活性及氨基酸特异性进行了研究。将Pto和Fen基因融合至麦芽糖结合蛋白的羧基末端，并在大肠杆菌中表达。在体外试验中，将纯化的融合蛋白与[γ-32P]ATP一起温育，结果表明这两种蛋白都能够进行自身磷酸化。亚结构域II中保守赖氨酸残基被替换为谷氨酰胺的突变融合蛋白无法进行自身磷酸化。对活性融合蛋白的磷酸氨基酸分析表明，这两种激酶都能磷酸化丝氨酸和苏氨酸残基，但不能磷酸化酪氨酸残基。