幽门螺杆菌对大环内酯类药物的耐药性：来自克拉霉素治疗患者菌株的机制与稳定性
Macrolide resistance in Helicobacter pylori: mechanism and stability in strains from clarithromycin-treated patients.
作者信息
Hultén K, Gibreel A, Sköld O, Engstrand L
机构信息
Department of Clinical Microbiology and Infectious Diseases, Uppsala University, Sweden.
出版信息
Antimicrob Agents Chemother. 1997 Nov;41(11):2550-3. doi: 10.1128/AAC.41.11.2550.
Abstract
Helicobacter pylori strains from seven patients treated with clarithromycin were investigated for development, mechanism, and stability of resistance. Genetic relatedness between pre- and posttreatment isolates was shown by arbitrary primed PCR. Clarithromycin resistance was associated with A-to-G transitions at either position 2143 or 2144 or at both positions 2116 and 2142. In four cases, the mutations were homozygous. The Cla(r) phenotype was stable after 50 subcultivations in vitro. No erythromycin-modifying enzymes or rRNA methylases were found by biological assays, PCR and sequencing, or cloning methods.
摘要
对7名接受克拉霉素治疗的患者的幽门螺杆菌菌株进行了耐药性的产生、机制及稳定性研究。通过任意引物PCR显示了治疗前后分离株之间的遗传相关性。克拉霉素耐药性与2143位或2144位的A到G转换或2116位和2142位同时出现的转换有关。在4例中，突变是纯合的。Cla(r)表型在体外传代50次后仍稳定。通过生物学检测、PCR及测序或克隆方法未发现红霉素修饰酶或rRNA甲基化酶。
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