Production of brefeldin-A.

作者信息

McCloud T G, Burns M P, Majadly F D, Muschik G M, Miller D A, Poole K K, Roach J M, Ross J T, Lebherz W B

机构信息

Chemical Synthesis and Analysis Laboratory, National Cancer Institute-Frederick Cancer Research and Development Center, Maryland 21702-1201, USA.

出版信息

J Ind Microbiol. 1995 Jul;15(1):5-9. doi: 10.1007/BF01570006.

Fermentation conditions are described for the production of the antitumor antibiotic 7-(S)-brefeldin-A (brefeldin-A) in liquid culture by Eupenicillium brefeldianum, (B.Dodge) Stolk and Scott, ATCC 58665. An analytical hplc method was developed which allowed rapid quantitation of the compound during fermentation. A kilogram of brefeldin-A was isolated from a fermentation at the 6800-liter scale.