乳糜泻患者外周血和肠道黏膜中的T细胞受体库

Lahat N, Ben-Nun A, Cohen L, Kinarty A, Lerner A

Department of Immunology and Serology, Carmel Medical Centre, Haifa, Israel.

Clin Exp Immunol. 1995 Sep;101(3):422-7. doi: 10.1111/j.1365-2249.1995.tb03129.x.

The alpha beta and gamma delta T cell receptor (TCR) repertoire in the peripheral blood and intestinal mucosa of six coeliac and six age-matched controls was analysed by reverse transcription and polymerase chain reaction (PCR). No TCR alpha and gamma delta restriction was observed in coeliacs and controls. However, V gamma 3 was expressed only in coeliac peripheral and intestinal T cells. V delta 2 was strongly expressed in coeliacs and scarcely transcribed in control cells. The unique expression of these gamma delta TCR in coeliac patients suggests that V gamma 3 and perhaps V delta 2 TCR-bearing lymphocytes may play a role in the pathogenesis of coeliac disease.

通过逆转录和聚合酶链反应（PCR）分析了6名乳糜泻患者和6名年龄匹配的对照者外周血和肠黏膜中的αβ和γδT细胞受体（TCR）库。在乳糜泻患者和对照者中未观察到TCRα和γδ限制。然而，Vγ3仅在乳糜泻患者的外周血和肠道T细胞中表达。Vδ2在乳糜泻患者中强烈表达，而在对照细胞中几乎不转录。这些γδTCR在乳糜泻患者中的独特表达表明，携带Vγ3以及可能携带Vδ2 TCR的淋巴细胞可能在乳糜泻疾病的发病机制中起作用。