Hellinger W C, Cawley J J, Alvarez S, Hogan S F, Harmsen W S, Ilstrup D M, Cockerill F R
Department of Internal Medicine, Mayo Clinic Jacksonville, Florida 32224, USA.
J Clin Microbiol. 1995 Jul;33(7):1787-90. doi: 10.1128/jcm.33.7.1787-1790.1995.
The performance characteristics of the Isolator (Wampole Laboratories, Cranbury, N.J.) and the BacT/Alert (Organon Teknika Corporation, Durham, N.C.) aerobic blood culture systems were compared for 6,009 blood culture sets obtained from patients with suspected bloodstream infections. The BacT/Alert aerobic bottle [BTA(O2)] was continuously agitated while it was incubated in 5% CO2 at 36 degrees C; culture plates prepared from the Isolator tube [I(O2)] were incubated in 5% CO2 at 37 degrees C. From 394 blood cultures, 416 clinically significant isolates of bacteria and yeasts were recovered. The overall yields for BTA(O2) and I(O2) were not significantly different (319 versus 336; P = 0.20). I(O2) recovered significantly more staphylococcus (P < 0.05) and yeast isolates (P < 0.01). BTA(O2) recovered significantly more aerobic and facultatively anaerobic gram-negative bacilli (P < 0.05). In blood culture sets which produced growth of the same organisms in both the BTA(O2) and I(O2) systems, the BTA(O2) system detected growth sooner, but more rapid identification was possible with the I(O2) system by virtue of earlier isolation of colonies on solid media.
对从疑似血流感染患者身上获取的6009份血培养标本，比较了隔绝器（新泽西州克兰伯里市万波实验室）和BacT/Alert（北卡罗来纳州达勒姆市奥加农泰尼克公司）需氧血培养系统的性能特征。BacT/Alert需氧瓶[BTA(O2)]在36℃的5%二氧化碳环境中孵育时持续搅拌；由隔绝器管制备的培养平板[I(O2)]在37℃的5%二氧化碳环境中孵育。从394份血培养标本中，分离出416株具有临床意义的细菌和酵母菌。BTA(O2)和I(O2)的总体培养阳性率无显著差异（319对336；P = 0.20）。I(O2)分离出的葡萄球菌（P < 0.05）和酵母菌（P < 0.01）显著更多。BTA(O2)分离出的需氧和兼性厌氧革兰氏阴性杆菌显著更多（P < 0.05）。在BTA(O2)和I(O2)系统中均培养出相同微生物的血培养标本中，BTA(O2)系统更早检测到生长，但由于I(O2)系统能更早地在固体培养基上分离出菌落，因此可以更快地进行鉴定。
