Verweij P E, Stynen D, Rijs A J, de Pauw B E, Hoogkamp-Korstanje J A, Meis J F
Department of Medical Microbiology, University Hospital Nijmegen, The Netherlands.
J Clin Microbiol. 1995 Jul;33(7):1912-4. doi: 10.1128/jcm.33.7.1912-1914.1995.
The performance of a direct sandwich enzyme-linked immunosorbent assay (ELISA) for detecting Aspergillus galactomannan was compared with that of the Pastorex Aspergillus antigen latex agglutination (LA) test by using 532 serum samples from 61 patients at risk for invasive aspergillosis. The ELISA gave positive results earlier in the course of infection than did the LA test. A sensitivity of 70% and a specificity of 86% were obtained for the LA test and corresponding values of 90 and 84% were obtained for the ELISA when a series of serum samples was employed.
通过使用来自61名有侵袭性曲霉病风险患者的532份血清样本,将用于检测曲霉半乳甘露聚糖的直接夹心酶联免疫吸附测定(ELISA)的性能与Pastorex曲霉抗原乳胶凝集(LA)试验的性能进行了比较。ELISA在感染过程中比LA试验更早给出阳性结果。当使用一系列血清样本时,LA试验的敏感性为70%,特异性为86%,ELISA的相应值分别为90%和84%。