用于诊断肺曲霉病的血清中PCR与抗原检测的比较。
Comparison between PCR and detection of antigen in sera for diagnosis of pulmonary aspergillosis.
作者信息
Kawamura S, Maesaki S, Noda T, Hirakata Y, Tomono K, Tashiro T, Kohno S
机构信息
Second Department of Internal Medicine, Nagasaki University School of Medicine, Nagasaki, Japan.
出版信息
J Clin Microbiol. 1999 Jan;37(1):218-20. doi: 10.1128/JCM.37.1.218-220.1999.
Abstract
We evaluated the usefulness of PCR and antigen detection for the diagnosis of pulmonary aspergillosis. Forty-four serum samples from patients with pulmonary aspergillosis (33 with pulmonary aspergilloma, 4 with allergic bronchopulmonary aspergillosis, 4 with invasive pulmonary aspergillosis, and 3 with aspergillus pyothorax) were used in this study. PCR detection of Aspergillus DNA in serum samples was successful in 39 patients. Galactomannan antigen was detected by sandwich enzyme-linked immunosorbent assay in 25 patients and by latex agglutination test in 13 patients. Detection of Aspergillus DNA in serum samples by nested PCR had the highest sensitivity of the three methods tested for the diagnosis of pulmonary aspergillosis.
摘要
我们评估了聚合酶链反应（PCR）和抗原检测在肺曲霉病诊断中的实用性。本研究使用了44份来自肺曲霉病患者的血清样本（33例肺曲菌球、4例变应性支气管肺曲霉病、4例侵袭性肺曲霉病和3例曲霉性脓胸）。血清样本中曲霉DNA的PCR检测在39例患者中获得成功。采用夹心酶联免疫吸附测定法在25例患者中检测到半乳甘露聚糖抗原，采用乳胶凝集试验在13例患者中检测到该抗原。在检测肺曲霉病的三种方法中，巢式PCR检测血清样本中的曲霉DNA具有最高的灵敏度。
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