Freyler H, Hofmann H, Schwab F
Albrecht Von Graefes Arch Klin Exp Ophthalmol. 1976 Feb 4;198(2):155-9. doi: 10.1007/BF00410015.
Between December 1973 and October 1974 scrapings of the corneal epithelium of 52 patients were examined by immunofluorescence. 41 of these corneal scrapings were suitable for examination. In 31 of 38 cases with the clinical diagnosis of a herpes simplex keratitis virus-specific antigen was found by means of immunofluorescence. The procedure takes only a few hours, and shows with 81,5% correct diagnoses a high reliability; scrapings of patients without herpetic affection never revealed a positive result. In 3 of 4 cases of deep herpetic keratitis, in which the herpetic etiology could only be supposed clinically, the diagnosis was proved by immunofluorescence.
1973年12月至1974年10月期间,对52例患者的角膜上皮刮片进行了免疫荧光检查。其中41例角膜刮片适合检查。在临床诊断为单纯疱疹性角膜炎的38例病例中,有31例通过免疫荧光法发现了病毒特异性抗原。该检查仅需数小时,诊断正确率达81.5%,可靠性高;无疱疹感染患者的刮片从未显示阳性结果。在4例深部疱疹性角膜炎病例中,临床仅怀疑为疱疹病因,经免疫荧光法证实了诊断。