Jacob P, Gopinathan U, Sharma S, Rao G N
Sight Savers' Corneal Training Centre, L. V. Prasad Eye Institute, Hyderabad, India.
Cornea. 1995 Jul;14(4):360-4. doi: 10.1097/00003226-199507000-00003.
A prospective comparative evaluation of Bard Parker blade no. 15 and calcium alginate swab in the collection of corneal material was conducted. The study group included 30 consecutive cases of bacterial (n = 17), fungal (n = 11), and mixed (n = 2) cases of microbial keratitis. The samples collected by blade and swab were processed in an identical manner for viable colony counts. The yields from blade and swab were compared using the proportion test, taking into consideration the mean counts of each organism. The swab yielded significantly greater growth than blade (p = 0.003) in mycotic ulcers. However, the two patients (2/11) who showed no growth by blade but were positive by swab had smears positive and were diagnosed as mycotic ulcers. The difference between the two methods was not significant for bacterial and mixed ulcers. Replacement of blade with calcium alginate swab did not appear to be advantageous for the investigation of infectious keratitis.
对巴德·帕克15号刀片和海藻酸钙拭子采集角膜材料进行了前瞻性比较评估。研究组包括30例连续的细菌性（n = 17）、真菌性（n = 11）和混合性（n = 2）微生物性角膜炎病例。用刀片和拭子采集的样本以相同方式处理以进行活菌计数。使用比例检验比较刀片和拭子的培养产量，并考虑每种微生物的平均计数。在真菌性溃疡中，拭子培养出的菌落显著多于刀片（p = 0.003）。然而，有两名患者（2/11），刀片培养无生长，但拭子培养呈阳性，涂片阳性，被诊断为真菌性溃疡。对于细菌性和混合性溃疡，两种方法的差异不显著。用海藻酸钙拭子替代刀片似乎对感染性角膜炎的检查并无优势。
