Armitstead P R, Gowland G
Gut. 1975 Dec;16(12):968-72. doi: 10.1136/gut.16.12.968.
The unseparated leucocytes, separated mononuclear cells and granulocytes of six control subjects and nine patients with colorectal cancer have been studied by a direct cell migration inhibition technique. A migratory index was calculated from the migration in the presence and absence of a perchloric acid extract of large bowel tumours. In 10% homologous AB serum, no significant migration inhibition occurred with any of the cells from control subjects. Five of the nine cancer patients showed significant inhibition with their unseparated leucocytes, seven of seven with their mononuclear cells, and none of nine with their granulocytes. In 10% autologous serum, some controls exhibited migration inhibition with their unseparated leucocytes and their granulocyte fraction, but not with the mononuclear cell fraction. Migration inhibition was also now apparent in the granulocyte fraction of the cancer patients. It is concluded that, with a soluble tumour antigen preparation, a mononuclear cell population increases the sensitivity of the direct migration inhibition test and that autologous serum may interfere directly with the migration of granulocytes, by an action not dependent upon the release of inhibitory factors from sensitized lymphocytes. This could explain some of the inconsistencies of the assay when using an unseparated leucocyte population.
采用直接细胞迁移抑制技术，对6名对照受试者和9名结肠直肠癌患者的未分离白细胞、分离的单核细胞和粒细胞进行了研究。根据在有无大肠肿瘤高氯酸提取物存在的情况下的迁移情况计算迁移指数。在10%的同源AB血清中，对照受试者的任何细胞均未出现明显的迁移抑制。9名癌症患者中有5名的未分离白细胞出现明显抑制，7名患者的单核细胞出现明显抑制，9名患者的粒细胞均未出现明显抑制。在10%的自体血清中，一些对照受试者的未分离白细胞及其粒细胞部分出现迁移抑制，但单核细胞部分未出现。癌症患者的粒细胞部分也出现了迁移抑制。结论是，使用可溶性肿瘤抗体制剂时，单核细胞群体可提高直接迁移抑制试验的敏感性，自体血清可能通过一种不依赖于致敏淋巴细胞释放抑制因子的作用直接干扰粒细胞的迁移。这可以解释在使用未分离白细胞群体时该检测方法的一些不一致之处。
