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小鼠脂多糖结合蛋白的纯化与特性分析

Purification and characterization of murine lipopolysaccharide-binding protein.

作者信息

Gallay P, Carrel S, Glauser M P, Barras C, Ulevitch R J, Tobias P S, Baumgartner J D, Heumann D

机构信息

Department of Internal Medicine, CHUV-Lausanne, Switzerland.

出版信息

Infect Immun. 1993 Feb;61(2):378-83. doi: 10.1128/iai.61.2.378-383.1993.

DOI:10.1128/iai.61.2.378-383.1993
PMID:7678583
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC302740/
Abstract

The serum protein lipopolysaccharide (LPS)-binding protein (LBP) seems to play an important role in regulating host responses to LPS. Complexes of LPS and LBP form in serum and stimulate monocytes, macrophages, or polymorphonuclear leukocytes after binding to CD14. Previous reports have described the structure and properties of LBP from human and rabbit sera. Since mice are used in some experimental models of endotoxemia or gram-negative bacterial infections, information is needed about the properties of murine LBP. Murine LBP was purified by ion-exchange chromatography and high-pressure liquid chromatography; its NH2-terminal sequence (TNPGLVTRIT) was very similar to those of human and rabbit LBPs (80 to 90% amino acid identity). Murine LBP resembled LBPs from other species in that it promoted the binding of LPS to monocytes and enhanced the sensitivity of monocytes to LPS at least 100-fold. Mouse LBP, like rabbit and human LBPs, was found to be an acute-phase protein. Further in vivo studies with mice and anti-CD14 or anti-LBP reagents should help determine the role of LBP in response to LPS challenges.

摘要

血清蛋白脂多糖（LPS）结合蛋白（LBP）似乎在调节宿主对LPS的反应中起重要作用。LPS与LBP的复合物在血清中形成，并在与CD14结合后刺激单核细胞、巨噬细胞或多形核白细胞。先前的报道描述了人血清和兔血清中LBP的结构和特性。由于小鼠被用于内毒素血症或革兰氏阴性细菌感染的一些实验模型中，因此需要有关鼠LBP特性的信息。通过离子交换色谱法和高压液相色谱法纯化鼠LBP；其NH2末端序列（TNPGLVTRIT）与人和兔LBP的序列非常相似（氨基酸同一性为80%至90%）。鼠LBP与其他物种的LBP相似，因为它促进LPS与单核细胞的结合，并将单核细胞对LPS的敏感性提高至少100倍。发现小鼠LBP与兔和人LBP一样，是一种急性期蛋白。使用小鼠以及抗CD14或抗LBP试剂进行的进一步体内研究应有助于确定LBP在应对LPS挑战中的作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d8e/302740/c364d6823d5c/iai00014-0021-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d8e/302740/c364d6823d5c/iai00014-0021-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d8e/302740/c364d6823d5c/iai00014-0021-a.jpg

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