正常血清和热灭活血清中脂多糖结合蛋白的免疫反应性及生物活性
Immunoreactivity and bioactivity of lipopolysaccharide-binding protein in normal and heat-inactivated sera.
作者信息
Mészáros K, Aberle S, White M, Parent J B
机构信息
Department of Sepsis Research, XOMA Corporation, Berkeley, California 94710.
出版信息
Infect Immun. 1995 Jan;63(1):363-5. doi: 10.1128/iai.63.1.363-365.1995.
Abstract
The lipopolysaccharide (LPS)-potentiating effect of serum is due to LPS-binding protein (LBP), which facilitates the binding of LPS to CD14 receptors. We observed a remarkable heat sensitivity of recombinant LBP and various sera with respect to both immunoreactivity (measured by enzyme-linked immunosorbent assay) and bioactivity (potentiation of LPS induction of tumor necrosis factor in monocytes). Human sera were more active and more heat sensitive than fetal bovine sera. The commonly practiced heat inactivation of human serum (56 degrees C, 30 min) resulted in a 70% loss of bioactivity, which caused an apparent decrease in the potency of LPS.
摘要
血清的脂多糖（LPS）增强作用归因于LPS结合蛋白（LBP），它促进LPS与CD14受体的结合。我们观察到重组LBP和各种血清在免疫反应性（通过酶联免疫吸附测定法测量）和生物活性（单核细胞中LPS诱导肿瘤坏死因子的增强作用）方面对热具有显著敏感性。人血清比胎牛血清更具活性且对热更敏感。人血清常用的热灭活方法（56℃，30分钟）导致生物活性丧失70%，这使得LPS的效力明显降低。
相似文献
1
Immunoreactivity and bioactivity of lipopolysaccharide-binding protein in normal and heat-inactivated sera.正常血清和热灭活血清中脂多糖结合蛋白的免疫反应性及生物活性
Infect Immun. 1995 Jan;63(1):363-5. doi: 10.1128/iai.63.1.363-365.1995.
2
A comparison of equine and bovine sera as sources of lipopolysaccharide-binding protein activity in equine monocytes incubated with lipopolysaccharide.将马血清和牛血清作为脂多糖结合蛋白活性来源，用于与脂多糖孵育的马单核细胞的比较。
Vet Immunol Immunopathol. 2008 Feb 15;121(3-4):275-80. doi: 10.1016/j.vetimm.2007.10.002. Epub 2007 Oct 10.
3
High concentrations of lipopolysaccharide-binding protein in serum of patients with severe sepsis or septic shock inhibit the lipopolysaccharide response in human monocytes.严重脓毒症或脓毒性休克患者血清中高浓度的脂多糖结合蛋白可抑制人单核细胞中的脂多糖反应。
Blood. 2001 Dec 15;98(13):3800-8. doi: 10.1182/blood.v98.13.3800.
4
5
Characterisation of bovine lipopolysaccharide binding protein and the in vivo acute phase response to Pasteurella haemolytica Type A.
Vet Immunol Immunopathol. 1995 Nov;49(1-2):61-74. doi: 10.1016/0165-2427(95)05458-i.
6
Bovine recombinant lipopolysaccharide binding protein (BRLBP) regulated apoptosis and inflammation response in lipopolysaccharide-challenged bovine mammary epithelial cells (BMEC).牛重组脂多糖结合蛋白（BRLBP）调节脂多糖刺激的牛乳腺上皮细胞（BMEC）中的细胞凋亡和炎症反应。
Mol Immunol. 2015 Jun;65(2):205-14. doi: 10.1016/j.molimm.2015.01.026. Epub 2015 Feb 18.
7
Influence of CD14, LBP and BPI in the monocyte response to LPS of different polysaccharide chain length.CD14、脂多糖结合蛋白（LBP）和杀菌/通透性增加蛋白（BPI）对不同多糖链长度的脂多糖（LPS）单核细胞反应的影响。
Scand J Immunol. 1995 Jul;42(1):119-27. doi: 10.1111/j.1365-3083.1995.tb03634.x.
8
CD14, a receptor for complexes of lipopolysaccharide (LPS) and LPS binding protein.
Science. 1990 Sep 21;249(4975):1431-3. doi: 10.1126/science.1698311.
9
10
Lipopolysaccharide-binding protein accelerates and augments Escherichia coli phagocytosis by alveolar macrophages.脂多糖结合蛋白可加速并增强肺泡巨噬细胞对大肠杆菌的吞噬作用。
J Surg Res. 2000 Dec;94(2):159-66. doi: 10.1006/jsre.2000.5975.
引用本文的文献
1
Modelling Human Gut-Microbiome Interactions in a 3D Bioelectronic Platform.在3D生物电子平台中模拟人类肠道微生物群相互作用
Small Sci. 2024 Apr 22;4(6):2300349. doi: 10.1002/smsc.202300349. eCollection 2024 Jun.
2
Myristoylated, alanine-rich C-kinase substrate (MARCKS) regulates toll-like receptor 4 signaling in macrophages.肉豆蔻酰化、富含丙氨酸的蛋白激酶 C 底物（MARCKS）调节巨噬细胞中 Toll 样受体 4 的信号转导。
Sci Rep. 2023 Nov 10;13(1):19562. doi: 10.1038/s41598-023-46266-x.
3
Prognostic Value and Daily Trend of Interleukin-6, Neutrophil CD64 Expression, C-Reactive Protein and Lipopolysaccharide-Binding Protein in Critically Ill Patients: Reliable Predictors of Outcome or Not?重症患者中白细胞介素-6、中性粒细胞CD64表达、C反应蛋白和脂多糖结合蛋白的预后价值及每日变化趋势：是否为可靠的预后预测指标？
J Med Biochem. 2015 Oct;34(4):431-439. doi: 10.1515/jomb-2015-0002. Epub 2015 Sep 19.
4
Yersinia pestis targets neutrophils via complement receptor 3.鼠疫耶尔森菌通过补体受体3靶向中性粒细胞。
Cell Microbiol. 2015 May;17(5):666-87. doi: 10.1111/cmi.12391. Epub 2014 Nov 25.
5
Selectivity analysis of single binder assays used in plasma protein profiling.用于血浆蛋白分析的单结合物分析方法的选择性分析。
Proteomics. 2013 Dec;13(23-24):3406-10. doi: 10.1002/pmic.201300030. Epub 2013 Nov 18.
6
Lipopolysaccharide binding protein is down-regulated during acute liver failure.脂多糖结合蛋白在急性肝衰竭时下调。
Dig Dis Sci. 2012 Apr;57(4):918-24. doi: 10.1007/s10620-012-2046-2. Epub 2012 Jan 26.
7
Peroxide-mediated chromatin remodelling of a nuclear factor kappa B site in the mouse inducible nitric oxide synthase promoter.过氧化物介导的小鼠诱导型一氧化氮合酶启动子中核因子κB位点的染色质重塑
Biochem J. 2004 Feb 1;377(Pt 3):809-18. doi: 10.1042/BJ20031235.
8
Involvement of CD14 and beta2-integrins in activating cells with soluble and particulate lipopolysaccharides and mannuronic acid polymers.CD14和β2整合素在通过可溶性和颗粒性脂多糖以及甘露糖醛酸聚合物激活细胞中的作用。
Infect Immun. 2000 Dec;68(12):6770-6. doi: 10.1128/IAI.68.12.6770-6776.2000.
9
Endotoxin, cytokines, and endotoxin binding proteins in obstructive jaundice and after preoperative biliary drainage.梗阻性黄疸及术前胆道引流后内毒素、细胞因子和内毒素结合蛋白的研究
Gut. 2000 May;46(5):725-31. doi: 10.1136/gut.46.5.725.
10
Increased circulating levels of lipopolysaccharide binding protein in children with Escherichia coli O157:H7 hemorrhagic colitis and hemolytic uremic syndrome.大肠杆菌O157:H7出血性结肠炎和溶血尿毒综合征患儿循环中脂多糖结合蛋白水平升高。
Clin Diagn Lab Immunol. 1999 Sep;6(5):773. doi: 10.1128/CDLI.6.5.773-773.1999.
本文引用的文献
1
Inhibition of lipopolysaccharide-associated endotoxin activities in vitro and in vivo by the human anti-lipid A monoclonal antibody SdJ5-1.17.15.人抗脂质A单克隆抗体SdJ5-1.17.15在体外和体内对脂多糖相关内毒素活性的抑制作用
Infect Immun. 1993 Sep;61(9):3873-8. doi: 10.1128/iai.61.9.3873-3878.1993.
2
Regulation of plasminogen activator inhibitor-1 levels in human monocytes.
Cell Immunol. 1993 Nov;152(1):7-17. doi: 10.1006/cimm.1993.1263.
3
Lipopolysaccharide (LPS)-binding protein is carried on lipoproteins and acts as a cofactor in the neutralization of LPS.脂多糖结合蛋白存在于脂蛋白上，并在脂多糖中和过程中作为辅助因子发挥作用。
J Exp Med. 1994 Sep 1;180(3):1025-35. doi: 10.1084/jem.180.3.1025.
4
Lipopolysaccharide-binding protein as a major plasma protein responsible for endotoxemic shock.脂多糖结合蛋白作为导致内毒素血症休克的主要血浆蛋白。
Proc Natl Acad Sci U S A. 1993 Nov 1;90(21):9935-8. doi: 10.1073/pnas.90.21.9935.
5
Endotoxin induces rapid protein tyrosine phosphorylation in 70Z/3 cells expressing CD14.内毒素可在表达CD14的70Z/3细胞中诱导快速的蛋白酪氨酸磷酸化。
J Biol Chem. 1993 Nov 25;268(33):25009-14.
6
Analysis of lipopolysaccharide binding by CD14.CD14对脂多糖结合的分析。
J Biol Chem. 1993 Nov 25;268(33):24818-23.
7
Recognition of bacterial endotoxins by receptor-dependent mechanisms.通过受体依赖性机制识别细菌内毒素。
Adv Immunol. 1993;53:267-89. doi: 10.1016/s0065-2776(08)60502-7.
8
Purification and characterization of murine lipopolysaccharide-binding protein.小鼠脂多糖结合蛋白的纯化与特性分析
Infect Immun. 1993 Feb;61(2):378-83. doi: 10.1128/iai.61.2.378-383.1993.
9
10
Serum components enhance bacterial lipopolysaccharide-induced tissue factor expression and tumor necrosis factor-alpha secretion by bovine alveolar macrophages in vitro.血清成分可增强细菌脂多糖在体外诱导牛肺泡巨噬细胞组织因子表达及肿瘤坏死因子-α分泌的能力。
J Leukoc Biol. 1994 Apr;55(4):483-8. doi: 10.1002/jlb.55.4.483.
Zotero 插件