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正常血清和热灭活血清中脂多糖结合蛋白的免疫反应性及生物活性

Immunoreactivity and bioactivity of lipopolysaccharide-binding protein in normal and heat-inactivated sera.

作者信息

Mészáros K, Aberle S, White M, Parent J B

机构信息

Department of Sepsis Research, XOMA Corporation, Berkeley, California 94710.

出版信息

Infect Immun. 1995 Jan;63(1):363-5. doi: 10.1128/iai.63.1.363-365.1995.

DOI:10.1128/iai.63.1.363-365.1995
PMID:7806380
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC173003/
Abstract

The lipopolysaccharide (LPS)-potentiating effect of serum is due to LPS-binding protein (LBP), which facilitates the binding of LPS to CD14 receptors. We observed a remarkable heat sensitivity of recombinant LBP and various sera with respect to both immunoreactivity (measured by enzyme-linked immunosorbent assay) and bioactivity (potentiation of LPS induction of tumor necrosis factor in monocytes). Human sera were more active and more heat sensitive than fetal bovine sera. The commonly practiced heat inactivation of human serum (56 degrees C, 30 min) resulted in a 70% loss of bioactivity, which caused an apparent decrease in the potency of LPS.

摘要

血清的脂多糖（LPS）增强作用归因于LPS结合蛋白（LBP），它促进LPS与CD14受体的结合。我们观察到重组LBP和各种血清在免疫反应性（通过酶联免疫吸附测定法测量）和生物活性（单核细胞中LPS诱导肿瘤坏死因子的增强作用）方面对热具有显著敏感性。人血清比胎牛血清更具活性且对热更敏感。人血清常用的热灭活方法（56℃，30分钟）导致生物活性丧失70%，这使得LPS的效力明显降低。

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