Cozzi R, Nicolai M, Perticone P, De Salvia R, Spuntarelli F
Dipartimento di Genetica e Biologia Molecolare, Università La Sapienza, Rome, Italy.
Mutat Res. 1993 Mar;299(1):37-44. doi: 10.1016/0165-1218(93)90117-v.
Humic acids are natural components of organic matter widespread in the environment. In spite of the incomplete knowledge about their composition, increasing interest in humic acid activity is justified by their ubiquity. Four different humic acids have been tested in Chinese hamster ovary cells in vitro, both alone and in combination with two well-known mutagens (mitomycin C and maleic hydrazide). Data about sister-chromatid exchanges, mitotic and proliferation indices were collected. Our results, on the whole, indicate: (i) a slight mutagenicity and toxicity of tested humic acids, probably due to chlorination during sample preparation; (ii) a desmutagenic rather than antimutagenic activity of the tested humic acids.
腐殖酸是环境中广泛存在的有机物质的天然成分。尽管对其组成的了解尚不完整，但由于它们的普遍存在，对腐殖酸活性的兴趣日益增加是合理的。已在中国仓鼠卵巢细胞中对四种不同的腐殖酸进行了体外测试，单独测试以及与两种著名的诱变剂（丝裂霉素C和顺丁烯二酸酰肼）联合测试。收集了有关姐妹染色单体交换、有丝分裂和增殖指数的数据。总体而言，我们的结果表明：（i）测试的腐殖酸具有轻微的诱变性和毒性，可能是由于样品制备过程中的氯化作用；（ii）测试的腐殖酸具有抗诱变而非抗诱变活性。
