绘制哺乳动物动粒内的DNA图谱。
Mapping DNA within the mammalian kinetochore.
作者信息
Cooke C A, Bazett-Jones D P, Earnshaw W C, Rattner J B
机构信息
Department of Anatomy, University of Calgary, Alberta, Canada.
出版信息
J Cell Biol. 1993 Mar;120(5):1083-91. doi: 10.1083/jcb.120.5.1083.
Abstract
The location of the cis-acting DNA sequences that direct the assembly of the mammalian kinetochore is not known. A variety of circumstantial evidence, however, has led to the widespread belief that they are present throughout the kinetochore including the kinetochore outer plate. To investigate this question directly, we have used two independent methods to localize DNA in and around the mammalian kinetochore. Both methods fail to reveal DNA in the outer kinetochore plate, finding instead that the outer-most detectable DNA in the centromere is located in the inner kinetochore plate. Our results imply that the outer kinetochore plate is primarily a proteinaceous structure. It is thus unlikely that fibers observed in the outer plate correspond to chromatin, as previously assumed. Our observations suggest that current models of kinetochore structure may need to be reconsidered.
摘要
指导哺乳动物动粒组装的顺式作用DNA序列的位置尚不清楚。然而，各种间接证据使人们普遍认为，它们存在于整个动粒中，包括动粒外板。为了直接研究这个问题，我们使用了两种独立的方法来定位哺乳动物动粒及其周围的DNA。两种方法都未能在外动粒板中发现DNA，而是发现着丝粒中最外层可检测到的DNA位于内动粒板中。我们的结果表明，外动粒板主要是蛋白质结构。因此，外板中观察到的纤维不太可能像以前假设的那样对应于染色质。我们的观察结果表明，目前的动粒结构模型可能需要重新考虑。
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本文引用的文献
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Structure of the colcemid-treated PtK1 kinetochore outer plate as determined by high voltage electron microscopic tomography.通过高压电子显微镜断层扫描确定的秋水仙酰胺处理的PtK1动粒外板结构。
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