Doğan A, MacDonald T T, Spencer J
Department of Histopathology, University College and Middlesex School of Medicine, London, UK.
Clin Exp Immunol. 1993 Mar;91(3):532-7. doi: 10.1111/j.1365-2249.1993.tb05937.x.
In this study we examined the distribution of the adhesion molecules ICAM-1, VCAM-1 and E-selectin in human fetal intestine, to determine whether they may have a role in the development of gut-associated lymphoid tissue. Secondly, we studied the tempo of induction of these molecules after T cell activation in explants of human fetal intestine cultured in vitro. In the fetus from 11 to 20 weeks gestation, endothelial expression of ICAM-1 and diffuse staining of VCAM-1 was observed in the lamina propria. In contrast, there was intense expression of ICAM-1 and VCAM-1 in the developing Peyer's patches, suggesting that these molecules may be involved in the accumulation or organization of lymphoid tissue in the gut. After T cell activation in fetal intestinal explants, the expression of ICAM-1 and VCAM-1 was increased on most endothelial cells, leucocytes, and stromal cells in the lamina propria. Expression was maintained for at least 4 days. In contrast, the induction of E-selectin was rapid, and the expression was transient, despite the continuing presence of activated T cells and macrophages. This suggests that other factors are required to prevent the down-regulation of E-selectin to maintain the sustained expression sometimes observed in vivo.
在本研究中，我们检测了黏附分子细胞间黏附分子-1（ICAM-1）、血管细胞黏附分子-1（VCAM-1）和E-选择素在人胎儿肠道中的分布，以确定它们是否可能在肠道相关淋巴组织的发育中发挥作用。其次，我们研究了体外培养的人胎儿肠道外植体经T细胞激活后这些分子的诱导进程。在妊娠11至20周的胎儿中，在固有层观察到ICAM-1的内皮表达和VCAM-1的弥漫性染色。相比之下，在发育中的派尔集合淋巴结中ICAM-1和VCAM-1有强烈表达，表明这些分子可能参与肠道淋巴组织的聚集或组织过程。胎儿肠道外植体经T细胞激活后，固有层中大多数内皮细胞、白细胞和基质细胞上ICAM-1和VCAM-1的表达增加。表达持续至少4天。相比之下。尽管活化的T细胞和巨噬细胞持续存在，E-选择素的诱导迅速且表达短暂。这表明需要其他因子来防止E-选择素的下调，以维持体内有时观察到的持续表达。
