Päällysaho T, Tervo K, Kivelä T, Virtanen I, Tarkkanen A, Tervo T
Department of Anatomy, University of Helsinki, Finland.
Graefes Arch Clin Exp Ophthalmol. 1993 Feb;231(2):61-5. doi: 10.1007/BF00920213.
An orbital nylon prosthesis was removed because of an infection caused by Staphylococcus aureus that was resistant to antimicrobials. It was processed for histopathology and immunohistochemistry. Within 3 weeks the implant had an extensive ingrowth of fibrovascular tissue containing chronic inflammatory cells, foreign body giant cells, and myofibroblasts. By using the indirect immunofluorescent method, this tissue was found to react with monoclonal antibodies (Mabs) against extradomain A of cellular fibronectin (EDA-cFN) and tenascin (TN). The presence of EDA-cFN and TN within the implant are indicative of an active healing process, since both of these proteins, scarce in adult tissues, have been shown to be reexpressed during tissue regeneration. The findings suggest that fibronectin plays a definite role in bacterial adherence and foreign body infections.
一个眼眶尼龙假体因感染金黄色葡萄球菌且该菌对抗菌药物耐药而被取出。对其进行了组织病理学和免疫组织化学处理。在3周内，植入物出现大量纤维血管组织向内生长，其中含有慢性炎症细胞、异物巨细胞和成肌纤维细胞。通过间接免疫荧光法发现，该组织与针对细胞纤连蛋白（EDA-cFN）和腱生蛋白（TN）的A结构域外区的单克隆抗体（Mabs）发生反应。植入物中存在EDA-cFN和TN表明愈合过程活跃，因为这两种在成年组织中稀缺的蛋白质已被证明在组织再生过程中会重新表达。这些发现表明纤连蛋白在细菌黏附和异物感染中起明确作用。
