Acsády L, Halasy K, Freund T F
Department of Functional Neuroanatomy, Hungarian Academy of Sciences, Budapest.
Neuroscience. 1993 Feb;52(4):829-41. doi: 10.1016/0306-4522(93)90532-k.
The subcortical innervation of a recently described subpopulation of non-pyramidal neurons, containing the calcium binding protein, calretinin, was investigated in the rat hippocampus using the anterograde tracer Phaseolus vulgaris-leucoagglutinin and double immunocytochemistry for calretinin and serotonin at the light and electron microscopic levels. Our results show that the GABAergic component of the septohippocampal pathway and the serotonergic raphe afferents establish multiple synaptic contacts with the calretinin-immunoreactive interneurons. The majority of the targets of both pathways were spine-free calretinin neurons known to innervate the dendritic region of the principal cells, but the GABAergic septal pathway was found to terminate also on the spiny neurons of stratum lucidum of the CA3 region and in the dentate hilus. The present results demonstrate that the serotonergic raphe-hippocampal and the GABAergic septohippocampal pathways are able to modulate dendritic inhibition of principal cells via calretinin-containing GABAergic interneurons.
利用顺行示踪剂菜豆凝集素以及在光镜和电镜水平上对钙视网膜蛋白和5-羟色胺进行双重免疫细胞化学，在大鼠海马体中研究了最近描述的含有钙结合蛋白钙视网膜蛋白的非锥体神经元亚群的皮质下神经支配。我们的结果表明，隔海马通路的GABA能成分和5-羟色胺能中缝传入纤维与钙视网膜蛋白免疫反应性中间神经元建立了多个突触联系。这两条通路的大多数靶点是已知支配主细胞树突区域的无棘钙视网膜蛋白神经元，但发现GABA能隔区通路也终止于CA3区透明层的棘状神经元和齿状回门区。目前的结果表明，5-羟色胺能中缝-海马通路和GABA能隔海马通路能够通过含钙视网膜蛋白的GABA能中间神经元调节主细胞的树突抑制。
