Schmued L C, Snavely L F
Division of Neurotoxicology, Food and Drug Administration, Jefferson, Arkansas 72079.
J Histochem Cytochem. 1993 May;41(5):777-82. doi: 10.1177/41.5.7682231.
Fluoro-Ruby, the fluorescent tetramethylrhodamine-dextran-amine used to demonstrate anterograde axon transport, has been successfully photoconverted and subsequently localized by electron microscopy. The photoconversion was accomplished by irradiating the tissue with green light while bathing it in a solution containing DAB. The tissue could then be examined by brightfield microscopy or processed for conventional electron microscopy. Potential advantages of the technique include greater permanence and contrast at the light microscopic level and the ability to resolve synaptic connectivity at the electron microscopic level.
荧光红宝石，一种用于显示顺行轴突运输的荧光四甲基罗丹明-葡聚糖-胺，已成功进行光转化，并随后通过电子显微镜进行定位。光转化是通过在含有二氨基联苯胺（DAB）的溶液中浸泡组织的同时用绿光照射组织来完成的。然后可以通过明场显微镜检查该组织，或将其处理用于传统电子显微镜检查。该技术的潜在优势包括在光学显微镜水平上具有更高的持久性和对比度，以及在电子显微镜水平上解析突触连接性的能力。
