一氧化氮合酶抑制剂可预防蓖麻油诱导的大鼠腹泻。

Inhibitors of nitric oxide synthetase prevent castor-oil-induced diarrhoea in the rat.

Mascolo N, Izzo A A, Barbato F, Capasso F

Department of Experimental Pharmacology, University of Naples Federico II, Italy.

Br J Pharmacol. 1993 Apr;108(4):861-4. doi: 10.1111/j.1476-5381.1993.tb13478.x.

Castor oil (2 ml orally) produced copious diarrhoea in rats 3 h after its administration. 2. Pretreatment (intraperitoneal, i.p.) of rats with the NO synthesis inhibitors NG-nitro-L-arginine methyl ester (L-NAME, 1-25 mg kg-1) and NG-monomethyl-L-arginine (L-NMMA, 2.5-100 mg kg-1) inhibited or prevented castor-oil-induced diarrhoea. L-Arginine (150-600 mg kg-1, i.p.) administered to rats pretreated with L-NAME 10 mg kg-1, drastically reduced the antidiarrhoeal activity of L-NAME in a dose-related manner. D-Arginine (900 mg kg-1) did not modify the protection by L-NAME. 3. Pretreatment (i.p.) of rats with L-NAME (2.5-25 mg kg-1) decreased the intestinal fluid accumulation and Na+ secretion induced by castor oil. L-Arginine (600 mg kg-1) but not D-arginine (900 mg kg-1) counteracted the inhibitory effect of L-NAME (10 mg kg-1). 4. L-NAME (10 and 25 mg kg-1) had no significant effect on the intestinal transit in normal rats or those given castor oil. 5. These results provide evidence that nitric oxide (NO) could play an important role in castor-oil-induced diarrhoea.

蓖麻油（口服2毫升）给药3小时后在大鼠中引起大量腹泻。2. 用一氧化氮合成抑制剂NG-硝基-L-精氨酸甲酯（L-NAME，1 - 25毫克/千克）和NG-单甲基-L-精氨酸（L-NMMA，2.5 - 100毫克/千克）对大鼠进行预处理（腹腔注射，i.p.）可抑制或预防蓖麻油诱导的腹泻。给用10毫克/千克L-NAME预处理的大鼠腹腔注射L-精氨酸（150 - 600毫克/千克），以剂量相关的方式显著降低了L-NAME的止泻活性。D-精氨酸（900毫克/千克）未改变L-NAME的保护作用。3. 用L-NAME（2.5 - 25毫克/千克）对大鼠进行预处理（腹腔注射）可减少蓖麻油诱导的肠液积聚和Na⁺分泌。L-精氨酸（600毫克/千克）而非D-精氨酸（900毫克/千克）抵消了L-NAME（10毫克/千克）的抑制作用。4. L-NAME（10和25毫克/千克）对正常大鼠或给予蓖麻油的大鼠的肠道转运没有显著影响。5. 这些结果提供了证据表明一氧化氮（NO）可能在蓖麻油诱导的腹泻中起重要作用。

Inhibitors of nitric oxide synthetase prevent castor-oil-induced diarrhoea in the rat.