卡介苗感染的人类单核细胞的识别与破坏。
Recognition and destruction of Bacillus Calmette-Guerin-infected human monocytes.
作者信息
Molloy A, Meyn P A, Smith K D, Kaplan G
机构信息
Department of Cellular Physiology and Immunology, Rockefeller University, New York, New York 10021.
出版信息
J Exp Med. 1993 Jun 1;177(6):1691-8. doi: 10.1084/jem.177.6.1691.
Abstract
We have established a long-term culture system to study macrophages chronically infected with mycobacteria. Monocytes are infected with Bacillus Calmette-Guerin (BCG) and support exponential intracellular replication without profound perturbation of normal host cell function. We have used this system to investigate lymphokine-activated killer (LAK)-mediated cytolysis. We have found that interleukin 2 stimulation of peripheral blood lymphocytes generates a cytotoxic activity against human monocytes. A CD56- subpopulation of LAK cells specifically recognizes and lyses BCG-infected cells. Lysis of the host cell has no effect on parasite viability and results in the liberation of bacteria capable of infecting more cells.
摘要
我们建立了一个长期培养系统，用于研究慢性感染分枝杆菌的巨噬细胞。单核细胞被卡介苗（BCG）感染，并支持其在细胞内呈指数级复制，而不会对正常宿主细胞功能造成严重干扰。我们利用这个系统研究淋巴因子激活的杀伤细胞（LAK）介导的细胞溶解作用。我们发现，白细胞介素2刺激外周血淋巴细胞可产生针对人单核细胞的细胞毒性活性。LAK细胞的CD56-亚群可特异性识别并裂解被BCG感染的细胞。宿主细胞的裂解对寄生虫的生存能力没有影响，反而会导致能够感染更多细胞的细菌释放出来。
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