分离包含位于编码链起始、中间或末端的结构基因的天然DNA片段。
Isolation of native DNA fragments containing structural genes at the beginning, in the middle or at the end of the coding strand.
作者信息
Yenikolopov G N, Ryskov A P, Nitta T, Georgiev G P
出版信息
Nucleic Acids Res. 1976 Oct;3(10):2645-63. doi: 10.1093/nar/3.10.2645.
Abstract
A method for the isolation of the DNA fragments containing structural genes at the beginning, in the middle and at the end of the coding strand is described. It involves mechanical or enzymatic shearing, exonuclease treatment, hybridization with mRNA and subsequent retention of DNA-mRNA complex on poly(U)-Sepharose. Hybridization of the DNA fragments with mRNA takes place with high specificity and isolated material is enriched in structural genes. Applications of this method for gene isolation are discussed.
摘要
本文描述了一种分离包含编码链起始、中间和末端结构基因的DNA片段的方法。该方法包括机械或酶切剪切、核酸外切酶处理、与mRNA杂交以及随后将DNA-mRNA复合物保留在聚（U）-琼脂糖上。DNA片段与mRNA的杂交具有高度特异性，分离得到的物质富含结构基因。文中还讨论了该方法在基因分离中的应用。
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Isolation of native DNA fragments containing structural genes at the beginning, in the middle or at the end of the coding strand.分离包含位于编码链起始、中间或末端的结构基因的天然DNA片段。
Nucleic Acids Res. 1976 Oct;3(10):2645-63. doi: 10.1093/nar/3.10.2645.
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