将合成珠蛋白基因整合到大肠杆菌质粒中。
Integration of synthetic globin genes into an E. coli plasmid.
作者信息
Wood K O, Lee J C
出版信息
Nucleic Acids Res. 1976 Aug;3(8):1961-71. doi: 10.1093/nar/3.8.1961.
Abstract
Rabbit globin mRNA has been purified and used as a template by reverse transcriptase. The resulting duplex molecule consisting of rabbit globin mRNA/cNDA has been linked in vitro to Eco RI cleaved plasmid Col E1 DNA. Transformation of E. coli C6OO by this recombinant molecule has been achieved. Transformed bacteria acquire the colicin EQ immunity of Col E1 and a closed circular DNA species of 4.40-4.45 x 10 (6) daltons in molecular weight, an increase of 2.0-2.5 x 10(5) daltons compared to that of the parent plasmid DNA. In addition , 3H cDNA synthesized from globin RNA hybridized perferentially to the recombinant plasmid DNA.
摘要
兔珠蛋白信使核糖核酸（mRNA）已被纯化，并用作逆转录酶的模板。由此产生的由兔珠蛋白mRNA/互补脱氧核糖核酸（cDNA）组成的双链分子已在体外与经Eco RI切割的质粒Col E1 DNA连接。已实现用这种重组分子转化大肠杆菌C600。转化后的细菌获得了Col E1的大肠杆菌素E1免疫性，以及一种分子量为4.40 - 4.45×10⁶道尔顿的闭环DNA种类，与亲本质粒DNA相比增加了2.0 - 2.5×10⁵道尔顿。此外，从珠蛋白RNA合成的³H cDNA优先与重组质粒DNA杂交。
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Proc Natl Acad Sci U S A. 1970 Sep;67(1):68-73. doi: 10.1073/pnas.67.1.68.
3
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