Taniguchi K, Urasawa T, Urasawa S, Yasuhara T
J Med Virol. 1984;14(2):115-25. doi: 10.1002/jmv.1890140205.
Nonneutralizing monoclonal antibodies were prepared against two strains, S2 and YO, of human rotaviruses isolated in cell culture. S2-37 and YO-5 antibodies had subgroup I and subgroup II specificities, respectively. The remaining antibodies (S2-65, YO-71, YO-89, and YO-156) reacted commonly with all the rotaviruses examined. All of the monoclonal antibodies agglutinated exclusively single-shelled particles and immunoprecipitated 42,000-dalton protein, a major component of inner capsid. Using the three monoclonal antibodies (S2-37, YO-5, and YO-156), an enzyme-linked immunosorbent assay was developed for detecting and subgrouping human rotavirus isolates.
针对在细胞培养中分离得到的两株人轮状病毒S2和YO制备了非中和性单克隆抗体。S2 - 37和YO - 5抗体分别具有I亚组和II亚组特异性。其余抗体（S2 - 65、YO - 71、YO - 89和YO - 156）与所有检测的轮状病毒均有共同反应。所有单克隆抗体仅凝集单壳颗粒，并免疫沉淀42,000道尔顿蛋白，这是内衣壳的主要成分。利用三种单克隆抗体（S2 - 37、YO - 5和YO - 156）开发了一种酶联免疫吸附测定法，用于检测人轮状病毒分离株并进行亚组分型。
