Fukunaga M, Sohnaka M, Yanagihara Y
Faculty of Pharmacy and Pharmaceutical Sciences, University of Fukuyama, Hiroshima, Japan.
J Gen Microbiol. 1993 Jun;139 Pt 6:1141-6. doi: 10.1099/00221287-139-6-1141.
We investigated the usefulness of rRNA gene restriction fragment length polymorphism (RFLP) for grouping of the Borrelia isolates associated with Lyme disease or from ixodid ticks. Genomic DNA was digested with a restriction enzyme, blotted and hybridized with an rrl (23S rRNA) gene probe. The sizes of the restriction bands showed a good correlation with the genotypes recently proposed, and Borrelia isolates of diverse geographic origin formed four distinct DNA groups. Group I included all of the USA isolates and some European isolates; group II contained European isolates and Asian isolates; group III comprised European and Asian isolates; group IV included Japanese isolates and an eastern Russian isolate. Groups I, II and III corresponded to Borrelia burgdorferi, B. garinii and group VS461, respectively. The RFLPs of Japanese isolates were rather divergent and some of the isolates were quite distinct from the USA and European isolates. RFLP analysis of the rRNA genes and flanking regions, using rrl gene probes as we reported here, may be useful in the taxonomic study of Borrelia.
我们研究了rRNA基因限制性片段长度多态性（RFLP）在对与莱姆病相关的或来自硬蜱的疏螺旋体分离株进行分组方面的实用性。基因组DNA用一种限制性酶消化，印迹后与一个rrl（23S rRNA）基因探针杂交。限制性条带的大小与最近提出的基因型显示出良好的相关性，不同地理来源的疏螺旋体分离株形成了四个不同的DNA组。第一组包括所有美国分离株和一些欧洲分离株；第二组包含欧洲分离株和亚洲分离株；第三组由欧洲和亚洲分离株组成；第四组包括日本分离株和一个俄罗斯东部分离株。第一组、第二组和第三组分别对应于伯氏疏螺旋体、伽氏疏螺旋体和VS461组。日本分离株的RFLP相当不同，其中一些分离株与美国和欧洲分离株明显不同。如我们在此报道的，使用rrl基因探针对rRNA基因及其侧翼区域进行RFLP分析，可能有助于疏螺旋体的分类学研究。
