Sato Y, Miyamoto K, Iwaki A, Masuzawa T, Yanagihara Y, Korenberg E I, Gorelova N B, Volkov V I, Ivanov L I, Liberova R N
Department of Parasitology, Asahikawa Medical College, Hokkaido, Japan.
Appl Environ Microbiol. 1996 Oct;62(10):3887-9. doi: 10.1128/aem.62.10.3887-3889.1996.
Borrelia spirochetes were isolated from the adult ixodid tick (Ixodes persulcatus) in three areas of far eastern Russia, namely, Khabarovsk, Vladivostok, and Yuzhno-Sakhalinsk. Borrelia infective rates of ticks in those areas were 24.5, 41.4, and 25.1%, respectively (total rate was 26.6%). Spirochetes were also isolated from the tissues of small mammals captured at Khabarovsk (infective rate was 20.8%). Samples were classified by rRNA gene restriction fragment length polymorphism (RFLP) analysis. The isolated spirochetes from ticks were classified mainly RFLP ribotype group IV (B. garinii), followed by groups II (B. garinii), III (B. afzelii), and V (B. garinii), showing that B. garinii is a dominant species among them. Both B. garinii and B. afzelii were also found in rodents, and multiple infections with those two species were observed in some rodents. B. burgdorferi sensu stricto (group I) was not isolated from either ticks or rodents.
在俄罗斯远东地区的三个地方，即哈巴罗夫斯克、符拉迪沃斯托克和南萨哈林斯克，从成年硬蜱（全沟硬蜱）中分离出了疏螺旋体。这些地区蜱的疏螺旋体感染率分别为24.5%、41.4%和25.1%（总感染率为26.6%）。在哈巴罗夫斯克捕获的小型哺乳动物组织中也分离出了螺旋体（感染率为20.8%）。通过rRNA基因限制性片段长度多态性（RFLP）分析对样本进行分类。从蜱中分离出的螺旋体主要分类为RFLP核糖体型IV组（伽氏疏螺旋体），其次是II组（伽氏疏螺旋体）、III组（阿氏疏螺旋体）和V组（伽氏疏螺旋体），这表明伽氏疏螺旋体是其中的优势种。在啮齿动物中也发现了伽氏疏螺旋体和阿氏疏螺旋体，并且在一些啮齿动物中观察到了这两个物种的多重感染。狭义伯氏疏螺旋体（I组）未从蜱或啮齿动物中分离出来。
