Baranton G, Postic D, Saint Girons I, Boerlin P, Piffaretti J C, Assous M, Grimont P A
Unité de Bactériologie Moléculaire et Médicale, Institut Pasteur, Paris, France.
Int J Syst Bacteriol. 1992 Jul;42(3):378-83. doi: 10.1099/00207713-42-3-378.
We studied 48 Borrelia isolates that were associated with Lyme borreliosis or were isolated from ticks and identified three DNA relatedness groups by using the S1 nuclease method. The three DNA groups (genospecies) were associated with specific rRNA gene restriction patterns, protein electrophoresis patterns, and patterns of reactivity with murine monoclonal antibodies. Genospecies I corresponded to Borrelia burgdorferi sensu stricto since it contained the type strain of this species (strain ATCC 35210); this genospecies included 28 isolates from Europe and the United States. Genospecies II was named Borrelia garinii sp. nov. and included 13 isolates from Europe and Japan. Genospecies III (group VS461) included seven isolates from Europe and Japan.
我们研究了48株与莱姆病相关或从蜱虫中分离出的疏螺旋体菌株，并使用S1核酸酶法鉴定出三个DNA相关性组。这三个DNA组（基因种）与特定的rRNA基因限制性图谱、蛋白质电泳图谱以及与鼠单克隆抗体的反应模式相关。基因种I对应狭义的伯氏疏螺旋体，因为它包含该物种的模式菌株（菌株ATCC 35210）；该基因种包括来自欧洲和美国的28株分离株。基因种II被命名为伽氏疏螺旋体新种，包括来自欧洲和日本的13株分离株。基因种III（VS461组）包括来自欧洲和日本的7株分离株。
