十二指肠内盐酸对麻醉大鼠胰腺外分泌、门静脉促胰液素及血管活性肠肽血浆水平的剂量反应效应。

Dose-response effect of intraduodenal HCl on exocrine pancreatic secretion, portal secretin, and VIP plasma levels in anesthetized rats.

作者信息

García L J, Minguela A, Calvo J J, López M A

机构信息

Department of Physiology and Pharmacology, Faculty of Biology, University of Salamanca, Spain.

出版信息

Arch Int Physiol Biochim Biophys. 1993 May-Jun;101(3):167-71. doi: 10.3109/13813459309046470.

DOI:10.3109/13813459309046470

PMID:7691208

Abstract

In anaesthetized rats, a dose-dependent effect of different intraduodenal HCl loads (0.6, 1.2, 6 and 12 mmol/h) on exocrine pancreatic secretion and plasma levels of secretin and VIP has been observed. The flow rate of pancreatic juice showed a significant increase at all the doses used; the integrated percentage response (IPR) of flow were 359%, 595%, 894% and 1371% for, respectively, 0.6, 1.2, 6 and 12 mmol/h. The increase of protein output only achieved statistical significance at HCl loads of 6 mmol/h or more; the IPR of protein output were 153%, 305%, 320% and 420% for, respectively, 0.6, 1.2, 6 and 12 mmol/h HCl. Portal levels of secretin and VIP were measured by specific radioimmunoassays. Significant increase of secretin concentration was observed at HCl loads of 1.2 mmol/h or more, whilst VIP levels only increased significantly after intraduodenal HCl load of 6 mmol/h or more. The IPR of secretin release were 180%, 203%, 280% and 322% for, respectively, 0.6, 1.2, 6 and 12 mmol/h HCl and IPR of VIP release were 3%, 20%, 244% and 315% for, respectively, 0.6, 1.2, 6 and 12 mmol/h HCl. Our results suggest that secretin may play a more prominent role than VIP in stimulating exocrine pancreatic secretion in response to intraduodenal loads of acid in this species.

摘要

在麻醉大鼠中，已观察到不同十二指肠内盐酸负荷量（0.6、1.2、6和12 mmol/h）对外分泌性胰腺分泌以及血浆中促胰液素和血管活性肠肽水平的剂量依赖性效应。在所使用的所有剂量下，胰液流速均显著增加；0.6、1.2、6和12 mmol/h时的流量综合百分比反应（IPR）分别为359%、595%、894%和1371%。仅在盐酸负荷量为6 mmol/h或更高时，蛋白质输出量的增加才具有统计学意义；0.6、1.2、6和12 mmol/h盐酸负荷量时的蛋白质输出IPR分别为153%、305%、320%和420%。通过特异性放射免疫测定法测量门静脉中促胰液素和血管活性肠肽的水平。在盐酸负荷量为1.2 mmol/h或更高时，观察到促胰液素浓度显著增加，而血管活性肠肽水平仅在十二指肠内盐酸负荷量为6 mmol/h或更高时才显著增加。0.6、1.2、6和12 mmol/h盐酸负荷量时促胰液素释放的IPR分别为180%、203%、280%和322%，血管活性肠肽释放的IPR分别为3%、20%、244%和315%。我们的结果表明，在该物种中，响应十二指肠内酸负荷刺激外分泌性胰腺分泌时，促胰液素可能比血管活性肠肽发挥更突出的作用。