Yasuda T, Ogawa M, Murata A, Ohmachi Y, Yasuda T, Mori T, Matsubara K
Department of Surgery II, Osaka University Medical School, Japan.
Gene. 1993 Sep 15;131(2):275-80. doi: 10.1016/0378-1119(93)90306-n.
Pancreatic secretory trypsin inhibitor (PSTI) has been suggested to be an acute-phase reactant in humans and to be induced by inflammatory cytokines such as the interleukins IL-1 and IL-6. We report that PSTI is synthesized in hepatoma cells and that the gene expression is augmented by IL-6. The start points (tsp) for basal and augmented transcription are exactly the same as the tsp in normal pancreas. Analysis of the PSTI gene revealed that a 40-bp DNA fragment located between kb -3.84 and -3.80 carries the element responsible for both transcriptional activity and IL-6-induced gene expression. This 40-bp fragment contains TTGNNGNAATG, the consensus sequence for the NF-IL6-binding site, which is also known as the IL-6-responsive element that is conserved among various acute-phase genes. The basal activity was augmented by another sequence that lies between kb -4.0 and -3.9.
胰腺分泌性胰蛋白酶抑制剂（PSTI）被认为是人类的一种急性期反应物，并可由白细胞介素IL-1和IL-6等炎性细胞因子诱导产生。我们报告PSTI在肝癌细胞中合成，且其基因表达被IL-6增强。基础转录和增强转录的起始点（tsp）与正常胰腺中的tsp完全相同。对PSTI基因的分析表明，位于-3.84 kb和-3.80 kb之间的一个40 bp的DNA片段携带负责转录活性和IL-6诱导基因表达的元件。这个40 bp的片段包含TTGNNGNAATG，即NF-IL6结合位点的共有序列，它也被称为在各种急性期基因中保守的IL-6反应元件。基础活性被位于-4.0 kb和-3.9 kb之间的另一个序列增强。
