Close T J, Fenton R D, Moonan F
Department of Botany and Plant Sciences, University of California, Riverside 92521.
Plant Mol Biol. 1993 Oct;23(2):279-86. doi: 10.1007/BF00029004.
Dehydrins are characterized by the consensus KIKEKLPG amino acid sequence found near the carboxy terminus, and usually repeated from one to many times within the protein. A synthetic peptide containing this consensus sequence was used to produce specific antibodies that recognize dehydrins in a wide range of plants. This range covered two families of monocots, viz. Gramineae (Hordeum vulgare L., Triticum aestivum L., Zea mays L., Oryza sativa L.) and Liliaceae (Allium sativa L.), and five families of dicots, Malvaceae (Gossypium hirsutum L.), Solanaceae (Lycopersicon esculentum L.), Brassicaceae (Raphanus sativus L.), Fabaceae (Vigna unguiculata L.), and Cucurbitaceae (Cucumis sativus L.). Two families of gymnosperms, Pinaceae (Pinus edulis Engelm.) and Ginkgoaceae (Ginkgo biloba L.), were also included. For several plants in which dehydrin cDNA and genomic clones have previously been characterized, it now appears that the dehydrin family of proteins is larger, and the regulation of dehydrin expression much more complex, than earlier studies have shown.
脱水素的特征是在羧基末端附近发现的共有氨基酸序列KIKEKLPG，并且通常在蛋白质中重复一到多次。一种含有该共有序列的合成肽被用于制备特异性抗体，这些抗体能够识别多种植物中的脱水素。这个范围涵盖了两个单子叶植物科，即禾本科（大麦、小麦、玉米、水稻）和百合科（大蒜），以及五个双子叶植物科，锦葵科（陆地棉）、茄科（番茄）、十字花科（萝卜）、豆科（豇豆）和葫芦科（黄瓜）。还包括两个裸子植物科，松科（矮松）和银杏科（银杏）。对于几种先前已鉴定出脱水素cDNA和基因组克隆的植物，现在看来，脱水素蛋白家族比早期研究显示的更大，并且脱水素表达的调控要复杂得多。
