Sarkisov D S, Arefyeva A M, Panova N W
Institute of Developmental Biology, Russian Academy of Sciences, Moscow.
Eur J Histochem. 1993;37(3):199-206.
Two-wavelength scanning cytophotometry was performed for the measurement of DNA (Feulgen reaction) and total protein content (naphthol yellow S staining) in the same cardiac myocyte. A lack of proportionality between the DNA content (i.e, cell ploidy) and protein content has been detected in ventricles of normal weight in the human heart. While the ploidy classes contained ratios of 2:4:8:16:32, the ratio of the protein content was about 2:3:5:8:17. In hypertrophic left or right ventricles, the latter ratio composed a series of doubling. Owing to this additional growth, weight of muscle tissue may be increased from 30% to twice, depending on the myocyte ploidy of a given myocardium which varies from 4 to 10c on average under normal circumstances and to 30c in some cases of hypertrophy (where c is the haploid DNA content, and, accordingly, a haploid chromosome set). Thus, one of the mechanisms of cardiac hypertrophy has been revealed which comprises the first step of the growth and often the process per se. Polyploidization of cardiac myocytes and their postmitotic growth enhance the heart weight in normal circumstances and creates a growth reserve for hypertrophy under conditions of pathology in future.
采用双波长扫描细胞光度法在同一条心肌细胞中测量DNA（福尔根反应）和总蛋白含量（萘酚黄S染色）。在正常体重的人心室中，已检测到DNA含量（即细胞倍性）与蛋白质含量之间缺乏比例关系。虽然倍性类别包含2:4:8:16:32的比例，但蛋白质含量的比例约为2:3:5:8:17。在肥厚的左心室或右心室中，后一比例构成了一系列加倍。由于这种额外的生长，肌肉组织的重量可能会增加30%至两倍，这取决于给定心肌的心肌细胞倍性，在正常情况下平均从4c变化到10c，在某些肥厚病例中可达30c（其中c是单倍体DNA含量，相应地，是一个单倍体染色体组）。因此，已揭示出心肌肥厚的一种机制，其包括生长的第一步且往往是生长过程本身。心肌细胞的多倍体化及其有丝分裂后生长在正常情况下会增加心脏重量，并在未来病理状态下为肥厚创造生长储备。
