Hamilton-Fairley D, Kiddy D, Anyaoku V, Koistinen R, Seppälä M, Franks S
Department of Obstetrics and Gynaecology, Imperial College of Science, Technology and Medicine, St Mary's Hospital Medical School, London, UK.
Clin Endocrinol (Oxf). 1993 Sep;39(3):363-7. doi: 10.1111/j.1365-2265.1993.tb02378.x.
We determined the relationship of short-term changes in circulating insulin concentrations, resulting from an oral glucose load, to those in both sex hormone binding globulin (SHBG) and insulin-like growth factor binding protein 1 (IGFBP-1) and assessed the effect of a short-term low calorie diet on the levels of SHBG and IGFBP-1 during an oral glucose tolerance test.
A within-group comparison of biochemical indices during an oral glucose tolerance test before and after calorie restriction.
Six obese women with polycystic ovary syndrome with mean (SD) BMI 34.2 (3.4) kg/m2 were studied before and after one month on a very low calorie diet (350 kcal/day; Cambridge diet). Each subject was given a 75-g oral glucose load after an overnight fast and blood samples were taken every 30 minutes for 3 hours. These were analysed for glucose, insulin, SHBG, and IGFBP-1.
All the women lost weight (range 1.7-9.5 kg). The SHBG concentrations did not change significantly during the oral glucose tolerance test but there was a highly significant decline in IGFBP-1 levels both before (0 min, mean (SD) 27.3 (10.6); 180 min, 8.9 (4.2) micrograms/l) and after (0 min, 28.4 (12.1); 180 min, 6.2 (2.1) micrograms/l, P < 0.001) dieting. The sum of the SHBG concentrations during the test, however, was significantly lower prior (129.9 (40.5) nmol/l) to calorie restriction than after (164.3 (70.6) nmol/l), whereas there was no significant effect of dieting on the IGFBP-1 response to glucose.
The changes in insulin and SHBG concentrations found after dieting have been confirmed. SHBG levels, in contrast to IGFBP-1, do not change in response to a short-term increase in insulin or glucose concentrations. The difference in the response of the two binding proteins may be explained by differences in their half-lives in the circulation or the regulation of mRNA for the peptides by insulin. This study confirms that insulin regulates both SHBG and IGFBP-1 but that there is a difference in the time course of the response of the two proteins to insulin.
我们确定了口服葡萄糖负荷引起的循环胰岛素浓度短期变化与性激素结合球蛋白（SHBG）和胰岛素样生长因子结合蛋白1（IGFBP - 1）变化之间的关系，并评估了短期低热量饮食对口服葡萄糖耐量试验期间SHBG和IGFBP - 1水平的影响。
热量限制前后口服葡萄糖耐量试验期间生化指标的组内比较。
对6名患有多囊卵巢综合征的肥胖女性进行研究，她们的平均（标准差）体重指数为34.2（3.4）kg/m²，在接受极低热量饮食（350千卡/天；剑桥饮食）1个月前后进行观察。每位受试者在空腹过夜后给予75克口服葡萄糖负荷，并在3小时内每30分钟采集一次血样。对这些血样进行葡萄糖、胰岛素、SHBG和IGFBP - 1分析。
所有女性体重均减轻（范围为1.7 - 9.5千克）。口服葡萄糖耐量试验期间，SHBG浓度无显著变化，但节食前后IGFBP - 1水平均显著下降（节食前：0分钟时，平均（标准差）为27.（10.6）；180分钟时，为8.9（4.2）微克/升；节食后：0分钟时，为28.4（12.1）；180分钟时，为6.2（2.1）微克/升，P < 0.001）。然而，试验期间SHBG浓度总和在热量限制前（129.9（40.5）纳摩尔/升）显著低于热量限制后（（164.3（70.6）纳摩尔/升），而节食对IGFBP - 1对葡萄糖的反应无显著影响。
节食后胰岛素和SHBG浓度的变化得到证实。与IGFBP - 1不同，SHBG水平不会因胰岛素或葡萄糖浓度短期升高而改变。两种结合蛋白反应的差异可能是由于它们在循环中的半衰期不同，或胰岛素对肽段mRNA的调控不同。本研究证实胰岛素对SHBG和IGFBP - 1都有调节作用，但两种蛋白对胰岛素反应的时间进程存在差异。
