Xie C I, Lin R C, Antony V, Lumeng L, Li T K, Mai K, Liu C, Wang Q D, Zhao Z H, Wang G F
Department of Medicine, Indiana University School of Medicine, Indianapolis 46202.
Alcohol Clin Exp Res. 1994 Dec;18(6):1443-7. doi: 10.1111/j.1530-0277.1994.tb01448.x.
The extract from an edible vine, Pueraria lebata, has been reported to be efficacious in lessening alcohol intoxication. In this study, we have tested the efficacy of one of the major components, daidzin, from this plant extract. When ethanol (40% solution, 3 g/kg body weight) was given to fasted rats intragastrically, blood alcohol concentration (BAC) peaked at 30 min after alcohol ingestion and reached 1.77 +/- 0.14 mg/ml (mean values +/- SD, n = 6). If daidzin (30 mg/kg) was mixed with the ethanol solution and given to animals intragastrically, BAC was found to peak at 90 min after alcohol ingestion and reached only 1.20 +/- 0.30 mg/ml (n = 6) (p < 0.05 vs. controls). The ability of daidzin to delay and decrease peak BAC level after ethanol ingestion was also observed in fed animals. In both fasted and fed rats given alcohol without daidzin, BAC quickly declined after reaching its peak at 30 min. By contrast, BAC levels receded more slowly if daidzin was also fed to the animals. Daidzin showed a chronic effect. Rats fed daidzin for 7 days before ethanol challenge, but not on the day of challenge, also produced lower and later peak BAC levels. Interestingly, daidzin, whether fed to rats only once or chronically for 7 days, did not significantly alter activities of either alcohol dehydrogenase or mitochondrial aldehyde dehydrogenase in the liver. Further experiments demonstrated that daidzin shortened sleep time for rats receiving ethanol intragastrically (7 g/kg) but not intraperitoneally (2 g/kg). To test whether daidzin delayed stomach-emptying, [14C]polyethylene glycol was mixed with ethanol and fed to rats.(ABSTRACT TRUNCATED AT 250 WORDS)
据报道，食用藤本植物野葛的提取物在减轻酒精中毒方面具有功效。在本研究中，我们测试了该植物提取物的一种主要成分——大豆苷元的功效。当给禁食大鼠灌胃给予乙醇（40%溶液，3 g/kg体重）时，血液酒精浓度（BAC）在摄入酒精后30分钟达到峰值，为1.77±0.14 mg/ml（平均值±标准差，n = 6）。如果将大豆苷元（30 mg/kg）与乙醇溶液混合后给动物灌胃，发现BAC在摄入酒精后90分钟达到峰值，仅为1.20±0.30 mg/ml（n = 6）（与对照组相比，p < 0.05）。在喂食的动物中也观察到了大豆苷元延迟并降低乙醇摄入后BAC峰值水平的能力。在未给予大豆苷元而摄入酒精的禁食和喂食大鼠中，BAC在30分钟达到峰值后迅速下降。相比之下，如果也给动物喂食大豆苷元，BAC水平下降得更慢。大豆苷元显示出慢性效应。在乙醇激发前7天喂食大豆苷元的大鼠，而非在激发当天喂食的大鼠，也产生了更低且更晚出现的BAC峰值水平。有趣的是，无论给大鼠单次喂食还是连续7天长期喂食大豆苷元，都不会显著改变肝脏中酒精脱氢酶或线粒体乙醛脱氢酶的活性。进一步的实验表明，大豆苷元缩短了经胃给予乙醇（7 g/kg）但非经腹腔给予乙醇（2 g/kg）的大鼠的睡眠时间。为了测试大豆苷元是否延迟胃排空，将[14C]聚乙二醇与乙醇混合后喂给大鼠。（摘要截取自250字）
